<\/strong><\/span><\/h5>\n<\/strong><\/span><\/h5>\n<\/strong><\/span><\/h5>\n<\/strong><\/span><\/h5>\nProf. Dr. B\u0103descu Magda Mariana<\/strong><\/span>
Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fi<\/strong><\/span><\/h5>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ locked=”off”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.4.0″]<\/p>\n
Efectul antitumoral al unor peptide citotoxice antimicrobiene, cu aplicabilitate \u00een neoplaziile tegumentului<\/span>
\n Autori: Magda B\u0103descu, Manuela Ciocoiu, Daniela Jitaru, B.M. Diaconescu, Maria Cristina Vl\u0103deanu, Iris Bararu-Bojan, Carmen Anton, Codru\u021ba B\u0103descu<\/span><\/p>\nIntroducere: Cancerul este cea mai frecvent\u0103 cauz\u0103 a mortalit\u0103\u021bii umane la nivel mondial. Terapiile anticancer, cum ar fi chimioterapia \u0219i radia\u021biile, sunt asociate cu efecte secundare severe. Multe studii au ar\u0103tat c\u0103 unele peptidele antimicrobiene prezint\u0103 un spectru larg de activitate citotoxic\u0103 \u00eempotriva celulelor canceroase.
\nMaterial \u0219i metod\u0103: \u00cen acest studiu in vitro, am evaluat poten\u021bialul tumoricid al magaininei, cecropinei A \u0219i B, dermaseptinei \u0219i lycotoxinei asupra liniilor de celule tumorale: MDA-MB-231 (adenocarcinomul mamar) \u0219i M14K (mezoteliom uman) precum \u0219i asupra unei linii de keratinocite normale.
\nRezultate: Am analizat efectele acestor peptide cationice asupra viabilit\u0103\u021bii, citotoxicit\u0103\u021bii \u0219i prolifer\u0103rii celulelor din liniile celulare tumorale \u0219i keratinocite. Studiul de viabilitate a fost realizat folosind anexin\u0103 \u0219i 7-AAD (7-amino-actinomicin\u0103 D), pentru detectarea \u0219i m\u0103surarea apoptozei prin tehnica citometriei \u00een flux. Peptidele utilizate de noi sunt citolitice (antimicrobiene) \u0219i pot distruge integritatea membranei cu pierderea con\u021binutului celular \u0219i moartea celulelor. Rezultatele studiului nostru au relevat faptul c\u0103 efectele citotoxice ale peptidelor, asupra celulelor tumorale, depind de concentra\u021bia lor. Eficien\u021ba lor este maxim\u0103 la concentra\u021bii de 120 \u00b5M dar persist\u0103 \u0219i la concentra\u021bii de 60 \u00b5M. Efectele au fost nesemnificative la concentra\u021bii de 30 \u00b5M.. Peptidele studiate nu au avut efect citotoxic asupra keratinocitelor normale.
\nConcluzii: Intensitatea efectului tumoricid depinde at\u00e2t de natura peptidului utilizat \u015fi de concentra\u0163ia acestuia \u00een mediul de via\u0163\u0103 al celulelor, c\u00e2t \u015fi de tipul de linie celular\u0103 utilizat\u0103. Ele ar putea reprezenta o alternativ\u0103 terapeutic\u0103 asupra tumorilor maligne de piele.<\/p>\n
Antitumoral effect of some antimicrobial cytotoxic peptides, as a novel therapy against skin cancers<\/span>
\nAuthors: Magda B\u0103descu, Manuela Ciocoiu, Daniela Jitaru, B.M. Diaconescu, Maria Cristina Vl\u0103deanu, Iris Bararu-Bojan, Carmen Anton, Codru\u021ba B\u0103descu<\/span><\/p>\nIntroduction: Cancer is the most common cause of human mortality worldwide. Anticancer therapies, such as chemotherapy and radiation, are associated with severe side effects. Many studies have shown that some antimicrobial peptides have a cytotoxic activity against cancer cells.
\nMaterial and method: In this study, we evaluated the tumoricidal potential of magainin and cecropines (A and B) on tumor cell lines: MDA-MB-231 (breast adenocarcinoma) and M14K (human mesothelioma) as well as a normal keratinocyte lines.
\nResults: We analyzed the effects of these cationic peptides on viability, cytotoxicity and cell proliferation in tumor and keratinocyte cell lines. The feasibility study was performed using annexin and 7-AAD (7-amino-actinomycin D), for the detection and measurement of apoptosis by flow cytometry. The peptides used by us are cytolytic (antimicrobial) effect and can destroy the integrity of the membrane with loss of cell content and cell death. The results of our study revealed that the cytotoxic effects of peptides on tumor cells depend on their concentration. Their efficiency is maximum at concentrations of 120 \u00b5M but it persists at concentrations of 60 \u00b5M. The effects were insignificant at concentrations of 30 \u00b5M. The studied peptides had no cytotoxic effect on normal keratinocytes line.
\nConclusions: The intensity of the tumoricidal effect depends both on the nature of the peptide used and its concentration in the cell life environment, as well as on the type of tumoral cell line used. They could be a therapeutic alternative for malignant skin tumors.<\/p>\n
Scurt CV<\/span><\/p>\nProfesor Universitar
\n\u2022 Disciplina Fiziopatologie, Facultatea de Medicin\u0103, U.M.F. \u201eGrigore T. Popa\u201d Ia\u015fi
\n\u2022 Medic primar medicin\u0103 intern\u0103
\n\u2022 Membru a 15 societ\u0103\u0163i \u015ftiin\u0163ifice na\u0163ionale \u015fi interna\u0163ionale:
\nAutor a unui num\u0103r de 16 manuale didactice \u015fi a unui num\u0103r de 6 monografii, dintre care men\u0163ionez:
\n\u2022 Zincul, o speran\u0163\u0103 pentru diabetici (monografie) Magda B\u0103descu, Casa Editorial\u0103 “Demiurg” Ia\u015fi, 1997.
\n\u2022 Elemente Fiziopatologice de limbaj intercelular (Monografie), Manuela Ciocoiu, Magda B\u0103descu; Editura \u201cTerra Nostra\u201d, ISBN 973-85547-4-8, 2001.
\n\u2022 FIZIOPATOLOGIE. Insuficien\u0163a marilor sisteme func\u0163ionale, Manuela Ciocoiu, Magda B\u0103descu. Editura Junimea, Ia\u015fi, 2010, ISBN 978-973-37-1465-1
\n\u2022 Magda B\u0103descu, Manuela Ciocoiu, Lauren\u0163iu B\u0103descu. Natural polyphenols intake in experimental diabetes mellitus, LAP LAMBERT Academic Publishing 2012, ISBN-13: 978-3659211287
\n\u2022 Compendu de Fiziopatologie General\u0103; Magda B\u0103descu, Manuela Ciocoiu, Editura Vasiliana, ISBN 973-8148-23-5, Ia\u015fi, 2001.
\n\u2022 Compendu de Fiziopatologie Special\u0103; Magda B\u0103descu, Manuela Ciocoiu; Editura Vasiliana, ISBN 973-8148-32-4, Ia\u015fi, 2001.
\n\u2022 FIZIOPATOLOGIE SPECIAL\u0102, Editura \u201cGr. T. Popa\u201d, U.M.F. Ia\u015fi, 2011, coord. Magda B\u0103descu, ISBN 978-606-544-055-5
\nActivitatea \u015ftiin\u0163ific\u0103 se materializeaz\u0103 \u00een:
\n\u2022 31 lucr\u0103ri ISI publicate \u00een ultimii 10 ani
\n\u2022 66 lucr\u0103ri BDI publicate \u00een ultimii 10 ani
\n\u2022 37 lucr\u0103ri \u00een alte reviste, \u00een volumele unor conferin\u0163e cu ISBN sau ISSN
\n\u2022 11 programe de cercetare + 20 teze de doctorat finalizate si confirmate.
\nH-index Web of Science Core Collection = 9
\nFactor de impact cumulat (pentru toate articolele) = 42,48
\nTeme de cercetare de interes, cu aplicabilitate pentru studiile doctorale;
\n1. Patogenia complica\u021biilor diabetului zaharat precum \u0219i preven\u021bia \u0219i terapia acestora prin deriva\u021bi naturali (microelemente, polifenoli).
\n2. Posibilit\u0103\u021bi de limitare a complica\u021biilor hipertensiunii arteriale prin deriva\u021bi naturali.
\n3. Aspecte genetice \u0219i epigenetice \u00een diagnosticul \u0219i tratamentul cardiopatiei ischemice.
\n4. Aspecte celulare \u0219i moleculare \u00een etiopatogenia \u0219i terapia neoplazic\u0103.
\n5. Metode de diagnostic precoce \u0219i terapie \u00een bolile degenerative.<\/p>\n
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Contacteaz\u0103 operatorul PDI 2020<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@primaderma.ro\/2019″ submit_button_text=”Trimite mesajul” _builder_version=”3.19.4″ title_font=”||||||||”][et_pb_contact_field field_id=”Name” field_title=”Nume” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”Adresa de e-mail” field_type=”email” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Mesajul dvs.” field_type=”text” fullwidth_field=”on” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark”]<\/p>\n
Operatorul PDI 2020<\/p>\n
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<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAdresa: Str. A. Panu nr. 13, Iasi<\/span>
Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off”]<\/p>\n
Parteneri Media<\/p>\n
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Prof. Dr. B\u0103descu Magda Mariana Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fiEfectul antitumoral al unor peptide citotoxice antimicrobiene, cu aplicabilitate \u00een neoplaziile tegumentului Autori: Magda B\u0103descu, Manuela Ciocoiu, Daniela Jitaru, B.M. Diaconescu, Maria Cristina Vl\u0103deanu, Iris Bararu-Bojan, Carmen Anton, Codru\u021ba B\u0103descu Introducere: Cancerul este cea mai frecvent\u0103 cauz\u0103 a mortalit\u0103\u021bii umane la nivel […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-7478","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages\/7478","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/comments?post=7478"}],"version-history":[{"count":6,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages\/7478\/revisions"}],"predecessor-version":[{"id":8156,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages\/7478\/revisions\/8156"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/media?parent=7478"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/strong><\/span><\/h5>\n<\/strong><\/span><\/h5>\nProf. Dr. B\u0103descu Magda Mariana<\/strong><\/span>
Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fi<\/strong><\/span><\/h5>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ locked=”off”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.4.0″]<\/p>\n
Efectul antitumoral al unor peptide citotoxice antimicrobiene, cu aplicabilitate \u00een neoplaziile tegumentului<\/span>
\n Autori: Magda B\u0103descu, Manuela Ciocoiu, Daniela Jitaru, B.M. Diaconescu, Maria Cristina Vl\u0103deanu, Iris Bararu-Bojan, Carmen Anton, Codru\u021ba B\u0103descu<\/span><\/p>\nIntroducere: Cancerul este cea mai frecvent\u0103 cauz\u0103 a mortalit\u0103\u021bii umane la nivel mondial. Terapiile anticancer, cum ar fi chimioterapia \u0219i radia\u021biile, sunt asociate cu efecte secundare severe. Multe studii au ar\u0103tat c\u0103 unele peptidele antimicrobiene prezint\u0103 un spectru larg de activitate citotoxic\u0103 \u00eempotriva celulelor canceroase.
\nMaterial \u0219i metod\u0103: \u00cen acest studiu in vitro, am evaluat poten\u021bialul tumoricid al magaininei, cecropinei A \u0219i B, dermaseptinei \u0219i lycotoxinei asupra liniilor de celule tumorale: MDA-MB-231 (adenocarcinomul mamar) \u0219i M14K (mezoteliom uman) precum \u0219i asupra unei linii de keratinocite normale.
\nRezultate: Am analizat efectele acestor peptide cationice asupra viabilit\u0103\u021bii, citotoxicit\u0103\u021bii \u0219i prolifer\u0103rii celulelor din liniile celulare tumorale \u0219i keratinocite. Studiul de viabilitate a fost realizat folosind anexin\u0103 \u0219i 7-AAD (7-amino-actinomicin\u0103 D), pentru detectarea \u0219i m\u0103surarea apoptozei prin tehnica citometriei \u00een flux. Peptidele utilizate de noi sunt citolitice (antimicrobiene) \u0219i pot distruge integritatea membranei cu pierderea con\u021binutului celular \u0219i moartea celulelor. Rezultatele studiului nostru au relevat faptul c\u0103 efectele citotoxice ale peptidelor, asupra celulelor tumorale, depind de concentra\u021bia lor. Eficien\u021ba lor este maxim\u0103 la concentra\u021bii de 120 \u00b5M dar persist\u0103 \u0219i la concentra\u021bii de 60 \u00b5M. Efectele au fost nesemnificative la concentra\u021bii de 30 \u00b5M.. Peptidele studiate nu au avut efect citotoxic asupra keratinocitelor normale.
\nConcluzii: Intensitatea efectului tumoricid depinde at\u00e2t de natura peptidului utilizat \u015fi de concentra\u0163ia acestuia \u00een mediul de via\u0163\u0103 al celulelor, c\u00e2t \u015fi de tipul de linie celular\u0103 utilizat\u0103. Ele ar putea reprezenta o alternativ\u0103 terapeutic\u0103 asupra tumorilor maligne de piele.<\/p>\n
Antitumoral effect of some antimicrobial cytotoxic peptides, as a novel therapy against skin cancers<\/span>
\nAuthors: Magda B\u0103descu, Manuela Ciocoiu, Daniela Jitaru, B.M. Diaconescu, Maria Cristina Vl\u0103deanu, Iris Bararu-Bojan, Carmen Anton, Codru\u021ba B\u0103descu<\/span><\/p>\nIntroduction: Cancer is the most common cause of human mortality worldwide. Anticancer therapies, such as chemotherapy and radiation, are associated with severe side effects. Many studies have shown that some antimicrobial peptides have a cytotoxic activity against cancer cells.
\nMaterial and method: In this study, we evaluated the tumoricidal potential of magainin and cecropines (A and B) on tumor cell lines: MDA-MB-231 (breast adenocarcinoma) and M14K (human mesothelioma) as well as a normal keratinocyte lines.
\nResults: We analyzed the effects of these cationic peptides on viability, cytotoxicity and cell proliferation in tumor and keratinocyte cell lines. The feasibility study was performed using annexin and 7-AAD (7-amino-actinomycin D), for the detection and measurement of apoptosis by flow cytometry. The peptides used by us are cytolytic (antimicrobial) effect and can destroy the integrity of the membrane with loss of cell content and cell death. The results of our study revealed that the cytotoxic effects of peptides on tumor cells depend on their concentration. Their efficiency is maximum at concentrations of 120 \u00b5M but it persists at concentrations of 60 \u00b5M. The effects were insignificant at concentrations of 30 \u00b5M. The studied peptides had no cytotoxic effect on normal keratinocytes line.
\nConclusions: The intensity of the tumoricidal effect depends both on the nature of the peptide used and its concentration in the cell life environment, as well as on the type of tumoral cell line used. They could be a therapeutic alternative for malignant skin tumors.<\/p>\n
Scurt CV<\/span><\/p>\nProfesor Universitar
\n\u2022 Disciplina Fiziopatologie, Facultatea de Medicin\u0103, U.M.F. \u201eGrigore T. Popa\u201d Ia\u015fi
\n\u2022 Medic primar medicin\u0103 intern\u0103
\n\u2022 Membru a 15 societ\u0103\u0163i \u015ftiin\u0163ifice na\u0163ionale \u015fi interna\u0163ionale:
\nAutor a unui num\u0103r de 16 manuale didactice \u015fi a unui num\u0103r de 6 monografii, dintre care men\u0163ionez:
\n\u2022 Zincul, o speran\u0163\u0103 pentru diabetici (monografie) Magda B\u0103descu, Casa Editorial\u0103 “Demiurg” Ia\u015fi, 1997.
\n\u2022 Elemente Fiziopatologice de limbaj intercelular (Monografie), Manuela Ciocoiu, Magda B\u0103descu; Editura \u201cTerra Nostra\u201d, ISBN 973-85547-4-8, 2001.
\n\u2022 FIZIOPATOLOGIE. Insuficien\u0163a marilor sisteme func\u0163ionale, Manuela Ciocoiu, Magda B\u0103descu. Editura Junimea, Ia\u015fi, 2010, ISBN 978-973-37-1465-1
\n\u2022 Magda B\u0103descu, Manuela Ciocoiu, Lauren\u0163iu B\u0103descu. Natural polyphenols intake in experimental diabetes mellitus, LAP LAMBERT Academic Publishing 2012, ISBN-13: 978-3659211287
\n\u2022 Compendu de Fiziopatologie General\u0103; Magda B\u0103descu, Manuela Ciocoiu, Editura Vasiliana, ISBN 973-8148-23-5, Ia\u015fi, 2001.
\n\u2022 Compendu de Fiziopatologie Special\u0103; Magda B\u0103descu, Manuela Ciocoiu; Editura Vasiliana, ISBN 973-8148-32-4, Ia\u015fi, 2001.
\n\u2022 FIZIOPATOLOGIE SPECIAL\u0102, Editura \u201cGr. T. Popa\u201d, U.M.F. Ia\u015fi, 2011, coord. Magda B\u0103descu, ISBN 978-606-544-055-5
\nActivitatea \u015ftiin\u0163ific\u0103 se materializeaz\u0103 \u00een:
\n\u2022 31 lucr\u0103ri ISI publicate \u00een ultimii 10 ani
\n\u2022 66 lucr\u0103ri BDI publicate \u00een ultimii 10 ani
\n\u2022 37 lucr\u0103ri \u00een alte reviste, \u00een volumele unor conferin\u0163e cu ISBN sau ISSN
\n\u2022 11 programe de cercetare + 20 teze de doctorat finalizate si confirmate.
\nH-index Web of Science Core Collection = 9
\nFactor de impact cumulat (pentru toate articolele) = 42,48
\nTeme de cercetare de interes, cu aplicabilitate pentru studiile doctorale;
\n1. Patogenia complica\u021biilor diabetului zaharat precum \u0219i preven\u021bia \u0219i terapia acestora prin deriva\u021bi naturali (microelemente, polifenoli).
\n2. Posibilit\u0103\u021bi de limitare a complica\u021biilor hipertensiunii arteriale prin deriva\u021bi naturali.
\n3. Aspecte genetice \u0219i epigenetice \u00een diagnosticul \u0219i tratamentul cardiopatiei ischemice.
\n4. Aspecte celulare \u0219i moleculare \u00een etiopatogenia \u0219i terapia neoplazic\u0103.
\n5. Metode de diagnostic precoce \u0219i terapie \u00een bolile degenerative.<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”3.22″ background_color=”rgba(117,194,33,0.23)” background_image=”https:\/\/pdi.ro\/2020\/wp-content\/uploads\/2020\/02\/back_justflowers_overlay1.png” background_blend=”luminosity” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”97px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#75c221″ bottom_divider_flip=”vertical” bottom_divider_height_tablet=”” bottom_divider_height_phone=”59px” bottom_divider_height_last_edited=”on|desktop”][et_pb_row column_structure=”3_5,2_5″ _builder_version=”3.25″ custom_padding=”61px|0px|69px|0px|false|false”][et_pb_column type=”3_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” text_line_height=”1.2em” header_font=”||||||||” background_layout=”dark” text_font_size_tablet=”” text_font_size_phone=”” text_font_size_last_edited=”on|desktop”]<\/p>\n
Contacteaz\u0103 operatorul PDI 2020<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@primaderma.ro\/2019″ submit_button_text=”Trimite mesajul” _builder_version=”3.19.4″ title_font=”||||||||”][et_pb_contact_field field_id=”Name” field_title=”Nume” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”Adresa de e-mail” field_type=”email” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Mesajul dvs.” field_type=”text” fullwidth_field=”on” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark”]<\/p>\n
Operatorul PDI 2020<\/p>\n
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<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAdresa: Str. A. Panu nr. 13, Iasi<\/span>
Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off”]<\/p>\n
Parteneri Media<\/p>\n
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Prof. Dr. B\u0103descu Magda Mariana Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fiEfectul antitumoral al unor peptide citotoxice antimicrobiene, cu aplicabilitate \u00een neoplaziile tegumentului Autori: Magda B\u0103descu, Manuela Ciocoiu, Daniela Jitaru, B.M. Diaconescu, Maria Cristina Vl\u0103deanu, Iris Bararu-Bojan, Carmen Anton, Codru\u021ba B\u0103descu Introducere: Cancerul este cea mai frecvent\u0103 cauz\u0103 a mortalit\u0103\u021bii umane la nivel […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-7478","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages\/7478","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/comments?post=7478"}],"version-history":[{"count":6,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages\/7478\/revisions"}],"predecessor-version":[{"id":8156,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages\/7478\/revisions\/8156"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/media?parent=7478"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Prof. Dr. B\u0103descu Magda Mariana<\/strong><\/span>
Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fi<\/strong><\/span><\/h5>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ locked=”off”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.4.0″]<\/p>\n
Efectul antitumoral al unor peptide citotoxice antimicrobiene, cu aplicabilitate \u00een neoplaziile tegumentului<\/span>
\n Autori: Magda B\u0103descu, Manuela Ciocoiu, Daniela Jitaru, B.M. Diaconescu, Maria Cristina Vl\u0103deanu, Iris Bararu-Bojan, Carmen Anton, Codru\u021ba B\u0103descu<\/span><\/p>\nIntroducere: Cancerul este cea mai frecvent\u0103 cauz\u0103 a mortalit\u0103\u021bii umane la nivel mondial. Terapiile anticancer, cum ar fi chimioterapia \u0219i radia\u021biile, sunt asociate cu efecte secundare severe. Multe studii au ar\u0103tat c\u0103 unele peptidele antimicrobiene prezint\u0103 un spectru larg de activitate citotoxic\u0103 \u00eempotriva celulelor canceroase.
\nMaterial \u0219i metod\u0103: \u00cen acest studiu in vitro, am evaluat poten\u021bialul tumoricid al magaininei, cecropinei A \u0219i B, dermaseptinei \u0219i lycotoxinei asupra liniilor de celule tumorale: MDA-MB-231 (adenocarcinomul mamar) \u0219i M14K (mezoteliom uman) precum \u0219i asupra unei linii de keratinocite normale.
\nRezultate: Am analizat efectele acestor peptide cationice asupra viabilit\u0103\u021bii, citotoxicit\u0103\u021bii \u0219i prolifer\u0103rii celulelor din liniile celulare tumorale \u0219i keratinocite. Studiul de viabilitate a fost realizat folosind anexin\u0103 \u0219i 7-AAD (7-amino-actinomicin\u0103 D), pentru detectarea \u0219i m\u0103surarea apoptozei prin tehnica citometriei \u00een flux. Peptidele utilizate de noi sunt citolitice (antimicrobiene) \u0219i pot distruge integritatea membranei cu pierderea con\u021binutului celular \u0219i moartea celulelor. Rezultatele studiului nostru au relevat faptul c\u0103 efectele citotoxice ale peptidelor, asupra celulelor tumorale, depind de concentra\u021bia lor. Eficien\u021ba lor este maxim\u0103 la concentra\u021bii de 120 \u00b5M dar persist\u0103 \u0219i la concentra\u021bii de 60 \u00b5M. Efectele au fost nesemnificative la concentra\u021bii de 30 \u00b5M.. Peptidele studiate nu au avut efect citotoxic asupra keratinocitelor normale.
\nConcluzii: Intensitatea efectului tumoricid depinde at\u00e2t de natura peptidului utilizat \u015fi de concentra\u0163ia acestuia \u00een mediul de via\u0163\u0103 al celulelor, c\u00e2t \u015fi de tipul de linie celular\u0103 utilizat\u0103. Ele ar putea reprezenta o alternativ\u0103 terapeutic\u0103 asupra tumorilor maligne de piele.<\/p>\n
Antitumoral effect of some antimicrobial cytotoxic peptides, as a novel therapy against skin cancers<\/span>
\nAuthors: Magda B\u0103descu, Manuela Ciocoiu, Daniela Jitaru, B.M. Diaconescu, Maria Cristina Vl\u0103deanu, Iris Bararu-Bojan, Carmen Anton, Codru\u021ba B\u0103descu<\/span><\/p>\nIntroduction: Cancer is the most common cause of human mortality worldwide. Anticancer therapies, such as chemotherapy and radiation, are associated with severe side effects. Many studies have shown that some antimicrobial peptides have a cytotoxic activity against cancer cells.
\nMaterial and method: In this study, we evaluated the tumoricidal potential of magainin and cecropines (A and B) on tumor cell lines: MDA-MB-231 (breast adenocarcinoma) and M14K (human mesothelioma) as well as a normal keratinocyte lines.
\nResults: We analyzed the effects of these cationic peptides on viability, cytotoxicity and cell proliferation in tumor and keratinocyte cell lines. The feasibility study was performed using annexin and 7-AAD (7-amino-actinomycin D), for the detection and measurement of apoptosis by flow cytometry. The peptides used by us are cytolytic (antimicrobial) effect and can destroy the integrity of the membrane with loss of cell content and cell death. The results of our study revealed that the cytotoxic effects of peptides on tumor cells depend on their concentration. Their efficiency is maximum at concentrations of 120 \u00b5M but it persists at concentrations of 60 \u00b5M. The effects were insignificant at concentrations of 30 \u00b5M. The studied peptides had no cytotoxic effect on normal keratinocytes line.
\nConclusions: The intensity of the tumoricidal effect depends both on the nature of the peptide used and its concentration in the cell life environment, as well as on the type of tumoral cell line used. They could be a therapeutic alternative for malignant skin tumors.<\/p>\n
Scurt CV<\/span><\/p>\nProfesor Universitar
\n\u2022 Disciplina Fiziopatologie, Facultatea de Medicin\u0103, U.M.F. \u201eGrigore T. Popa\u201d Ia\u015fi
\n\u2022 Medic primar medicin\u0103 intern\u0103
\n\u2022 Membru a 15 societ\u0103\u0163i \u015ftiin\u0163ifice na\u0163ionale \u015fi interna\u0163ionale:
\nAutor a unui num\u0103r de 16 manuale didactice \u015fi a unui num\u0103r de 6 monografii, dintre care men\u0163ionez:
\n\u2022 Zincul, o speran\u0163\u0103 pentru diabetici (monografie) Magda B\u0103descu, Casa Editorial\u0103 “Demiurg” Ia\u015fi, 1997.
\n\u2022 Elemente Fiziopatologice de limbaj intercelular (Monografie), Manuela Ciocoiu, Magda B\u0103descu; Editura \u201cTerra Nostra\u201d, ISBN 973-85547-4-8, 2001.
\n\u2022 FIZIOPATOLOGIE. Insuficien\u0163a marilor sisteme func\u0163ionale, Manuela Ciocoiu, Magda B\u0103descu. Editura Junimea, Ia\u015fi, 2010, ISBN 978-973-37-1465-1
\n\u2022 Magda B\u0103descu, Manuela Ciocoiu, Lauren\u0163iu B\u0103descu. Natural polyphenols intake in experimental diabetes mellitus, LAP LAMBERT Academic Publishing 2012, ISBN-13: 978-3659211287
\n\u2022 Compendu de Fiziopatologie General\u0103; Magda B\u0103descu, Manuela Ciocoiu, Editura Vasiliana, ISBN 973-8148-23-5, Ia\u015fi, 2001.
\n\u2022 Compendu de Fiziopatologie Special\u0103; Magda B\u0103descu, Manuela Ciocoiu; Editura Vasiliana, ISBN 973-8148-32-4, Ia\u015fi, 2001.
\n\u2022 FIZIOPATOLOGIE SPECIAL\u0102, Editura \u201cGr. T. Popa\u201d, U.M.F. Ia\u015fi, 2011, coord. Magda B\u0103descu, ISBN 978-606-544-055-5
\nActivitatea \u015ftiin\u0163ific\u0103 se materializeaz\u0103 \u00een:
\n\u2022 31 lucr\u0103ri ISI publicate \u00een ultimii 10 ani
\n\u2022 66 lucr\u0103ri BDI publicate \u00een ultimii 10 ani
\n\u2022 37 lucr\u0103ri \u00een alte reviste, \u00een volumele unor conferin\u0163e cu ISBN sau ISSN
\n\u2022 11 programe de cercetare + 20 teze de doctorat finalizate si confirmate.
\nH-index Web of Science Core Collection = 9
\nFactor de impact cumulat (pentru toate articolele) = 42,48
\nTeme de cercetare de interes, cu aplicabilitate pentru studiile doctorale;
\n1. Patogenia complica\u021biilor diabetului zaharat precum \u0219i preven\u021bia \u0219i terapia acestora prin deriva\u021bi naturali (microelemente, polifenoli).
\n2. Posibilit\u0103\u021bi de limitare a complica\u021biilor hipertensiunii arteriale prin deriva\u021bi naturali.
\n3. Aspecte genetice \u0219i epigenetice \u00een diagnosticul \u0219i tratamentul cardiopatiei ischemice.
\n4. Aspecte celulare \u0219i moleculare \u00een etiopatogenia \u0219i terapia neoplazic\u0103.
\n5. Metode de diagnostic precoce \u0219i terapie \u00een bolile degenerative.<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”3.22″ background_color=”rgba(117,194,33,0.23)” background_image=”https:\/\/pdi.ro\/2020\/wp-content\/uploads\/2020\/02\/back_justflowers_overlay1.png” background_blend=”luminosity” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”97px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#75c221″ bottom_divider_flip=”vertical” bottom_divider_height_tablet=”” bottom_divider_height_phone=”59px” bottom_divider_height_last_edited=”on|desktop”][et_pb_row column_structure=”3_5,2_5″ _builder_version=”3.25″ custom_padding=”61px|0px|69px|0px|false|false”][et_pb_column type=”3_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” text_line_height=”1.2em” header_font=”||||||||” background_layout=”dark” text_font_size_tablet=”” text_font_size_phone=”” text_font_size_last_edited=”on|desktop”]<\/p>\n
Contacteaz\u0103 operatorul PDI 2020<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@primaderma.ro\/2019″ submit_button_text=”Trimite mesajul” _builder_version=”3.19.4″ title_font=”||||||||”][et_pb_contact_field field_id=”Name” field_title=”Nume” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”Adresa de e-mail” field_type=”email” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Mesajul dvs.” field_type=”text” fullwidth_field=”on” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark”]<\/p>\n
Operatorul PDI 2020<\/p>\n
[\/et_pb_text][et_pb_text _builder_version=”4.0.11″ text_font=”||||||||”]<\/p>\n
<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAdresa: Str. A. Panu nr. 13, Iasi<\/span>
Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off”]<\/p>\n
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Prof. Dr. B\u0103descu Magda Mariana Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fiEfectul antitumoral al unor peptide citotoxice antimicrobiene, cu aplicabilitate \u00een neoplaziile tegumentului Autori: Magda B\u0103descu, Manuela Ciocoiu, Daniela Jitaru, B.M. Diaconescu, Maria Cristina Vl\u0103deanu, Iris Bararu-Bojan, Carmen Anton, Codru\u021ba B\u0103descu Introducere: Cancerul este cea mai frecvent\u0103 cauz\u0103 a mortalit\u0103\u021bii umane la nivel […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-7478","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages\/7478","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/comments?post=7478"}],"version-history":[{"count":6,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages\/7478\/revisions"}],"predecessor-version":[{"id":8156,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages\/7478\/revisions\/8156"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/media?parent=7478"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\n Autori: Magda B\u0103descu, Manuela Ciocoiu, Daniela Jitaru, B.M. Diaconescu, Maria Cristina Vl\u0103deanu, Iris Bararu-Bojan, Carmen Anton, Codru\u021ba B\u0103descu<\/span><\/p>\n
Introducere: Cancerul este cea mai frecvent\u0103 cauz\u0103 a mortalit\u0103\u021bii umane la nivel mondial. Terapiile anticancer, cum ar fi chimioterapia \u0219i radia\u021biile, sunt asociate cu efecte secundare severe. Multe studii au ar\u0103tat c\u0103 unele peptidele antimicrobiene prezint\u0103 un spectru larg de activitate citotoxic\u0103 \u00eempotriva celulelor canceroase.
\nMaterial \u0219i metod\u0103: \u00cen acest studiu in vitro, am evaluat poten\u021bialul tumoricid al magaininei, cecropinei A \u0219i B, dermaseptinei \u0219i lycotoxinei asupra liniilor de celule tumorale: MDA-MB-231 (adenocarcinomul mamar) \u0219i M14K (mezoteliom uman) precum \u0219i asupra unei linii de keratinocite normale.
\nRezultate: Am analizat efectele acestor peptide cationice asupra viabilit\u0103\u021bii, citotoxicit\u0103\u021bii \u0219i prolifer\u0103rii celulelor din liniile celulare tumorale \u0219i keratinocite. Studiul de viabilitate a fost realizat folosind anexin\u0103 \u0219i 7-AAD (7-amino-actinomicin\u0103 D), pentru detectarea \u0219i m\u0103surarea apoptozei prin tehnica citometriei \u00een flux. Peptidele utilizate de noi sunt citolitice (antimicrobiene) \u0219i pot distruge integritatea membranei cu pierderea con\u021binutului celular \u0219i moartea celulelor. Rezultatele studiului nostru au relevat faptul c\u0103 efectele citotoxice ale peptidelor, asupra celulelor tumorale, depind de concentra\u021bia lor. Eficien\u021ba lor este maxim\u0103 la concentra\u021bii de 120 \u00b5M dar persist\u0103 \u0219i la concentra\u021bii de 60 \u00b5M. Efectele au fost nesemnificative la concentra\u021bii de 30 \u00b5M.. Peptidele studiate nu au avut efect citotoxic asupra keratinocitelor normale.
\nConcluzii: Intensitatea efectului tumoricid depinde at\u00e2t de natura peptidului utilizat \u015fi de concentra\u0163ia acestuia \u00een mediul de via\u0163\u0103 al celulelor, c\u00e2t \u015fi de tipul de linie celular\u0103 utilizat\u0103. Ele ar putea reprezenta o alternativ\u0103 terapeutic\u0103 asupra tumorilor maligne de piele.<\/p>\n
Antitumoral effect of some antimicrobial cytotoxic peptides, as a novel therapy against skin cancers<\/span> Introduction: Cancer is the most common cause of human mortality worldwide. Anticancer therapies, such as chemotherapy and radiation, are associated with severe side effects. Many studies have shown that some antimicrobial peptides have a cytotoxic activity against cancer cells. 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Diaconescu, Maria Cristina Vl\u0103deanu, Iris Bararu-Bojan, Carmen Anton, Codru\u021ba B\u0103descu Introducere: Cancerul este cea mai frecvent\u0103 cauz\u0103 a mortalit\u0103\u021bii umane la nivel […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-7478","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages\/7478","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/comments?post=7478"}],"version-history":[{"count":6,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages\/7478\/revisions"}],"predecessor-version":[{"id":8156,"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/pages\/7478\/revisions\/8156"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2020\/wp-json\/wp\/v2\/media?parent=7478"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nAuthors: Magda B\u0103descu, Manuela Ciocoiu, Daniela Jitaru, B.M. Diaconescu, Maria Cristina Vl\u0103deanu, Iris Bararu-Bojan, Carmen Anton, Codru\u021ba B\u0103descu<\/span><\/p>\n
\nMaterial and method: In this study, we evaluated the tumoricidal potential of magainin and cecropines (A and B) on tumor cell lines: MDA-MB-231 (breast adenocarcinoma) and M14K (human mesothelioma) as well as a normal keratinocyte lines.
\nResults: We analyzed the effects of these cationic peptides on viability, cytotoxicity and cell proliferation in tumor and keratinocyte cell lines. The feasibility study was performed using annexin and 7-AAD (7-amino-actinomycin D), for the detection and measurement of apoptosis by flow cytometry. The peptides used by us are cytolytic (antimicrobial) effect and can destroy the integrity of the membrane with loss of cell content and cell death. The results of our study revealed that the cytotoxic effects of peptides on tumor cells depend on their concentration. Their efficiency is maximum at concentrations of 120 \u00b5M but it persists at concentrations of 60 \u00b5M. The effects were insignificant at concentrations of 30 \u00b5M. The studied peptides had no cytotoxic effect on normal keratinocytes line.
\nConclusions: The intensity of the tumoricidal effect depends both on the nature of the peptide used and its concentration in the cell life environment, as well as on the type of tumoral cell line used. They could be a therapeutic alternative for malignant skin tumors.<\/p>\n
\n\u2022 Disciplina Fiziopatologie, Facultatea de Medicin\u0103, U.M.F. \u201eGrigore T. Popa\u201d Ia\u015fi
\n\u2022 Medic primar medicin\u0103 intern\u0103
\n\u2022 Membru a 15 societ\u0103\u0163i \u015ftiin\u0163ifice na\u0163ionale \u015fi interna\u0163ionale:
\nAutor a unui num\u0103r de 16 manuale didactice \u015fi a unui num\u0103r de 6 monografii, dintre care men\u0163ionez:
\n\u2022 Zincul, o speran\u0163\u0103 pentru diabetici (monografie) Magda B\u0103descu, Casa Editorial\u0103 “Demiurg” Ia\u015fi, 1997.
\n\u2022 Elemente Fiziopatologice de limbaj intercelular (Monografie), Manuela Ciocoiu, Magda B\u0103descu; Editura \u201cTerra Nostra\u201d, ISBN 973-85547-4-8, 2001.
\n\u2022 FIZIOPATOLOGIE. Insuficien\u0163a marilor sisteme func\u0163ionale, Manuela Ciocoiu, Magda B\u0103descu. Editura Junimea, Ia\u015fi, 2010, ISBN 978-973-37-1465-1
\n\u2022 Magda B\u0103descu, Manuela Ciocoiu, Lauren\u0163iu B\u0103descu. Natural polyphenols intake in experimental diabetes mellitus, LAP LAMBERT Academic Publishing 2012, ISBN-13: 978-3659211287
\n\u2022 Compendu de Fiziopatologie General\u0103; Magda B\u0103descu, Manuela Ciocoiu, Editura Vasiliana, ISBN 973-8148-23-5, Ia\u015fi, 2001.
\n\u2022 Compendu de Fiziopatologie Special\u0103; Magda B\u0103descu, Manuela Ciocoiu; Editura Vasiliana, ISBN 973-8148-32-4, Ia\u015fi, 2001.
\n\u2022 FIZIOPATOLOGIE SPECIAL\u0102, Editura \u201cGr. T. Popa\u201d, U.M.F. Ia\u015fi, 2011, coord. Magda B\u0103descu, ISBN 978-606-544-055-5
\nActivitatea \u015ftiin\u0163ific\u0103 se materializeaz\u0103 \u00een:
\n\u2022 31 lucr\u0103ri ISI publicate \u00een ultimii 10 ani
\n\u2022 66 lucr\u0103ri BDI publicate \u00een ultimii 10 ani
\n\u2022 37 lucr\u0103ri \u00een alte reviste, \u00een volumele unor conferin\u0163e cu ISBN sau ISSN
\n\u2022 11 programe de cercetare + 20 teze de doctorat finalizate si confirmate.
\nH-index Web of Science Core Collection = 9
\nFactor de impact cumulat (pentru toate articolele) = 42,48
\nTeme de cercetare de interes, cu aplicabilitate pentru studiile doctorale;
\n1. Patogenia complica\u021biilor diabetului zaharat precum \u0219i preven\u021bia \u0219i terapia acestora prin deriva\u021bi naturali (microelemente, polifenoli).
\n2. Posibilit\u0103\u021bi de limitare a complica\u021biilor hipertensiunii arteriale prin deriva\u021bi naturali.
\n3. Aspecte genetice \u0219i epigenetice \u00een diagnosticul \u0219i tratamentul cardiopatiei ischemice.
\n4. Aspecte celulare \u0219i moleculare \u00een etiopatogenia \u0219i terapia neoplazic\u0103.
\n5. Metode de diagnostic precoce \u0219i terapie \u00een bolile degenerative.<\/p>\n<\/span><\/p>\n
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