<\/strong><\/span><\/h5>\n<\/strong><\/span><\/h5>\n<\/strong><\/span><\/h5>\n<\/strong><\/span><\/h5>\nProf. Dr. B\u0103descu Magda Mariana<\/strong><\/span>
Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fi<\/strong><\/span><\/h5>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ locked=”off”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.6″]<\/p>\n
Peptidele antimicrobiene o speran\u021b\u0103 \u00een terapia melanomului malign<\/span>
\n Autori: Magda B\u0103descu1, Manuela Ciocoiu1, Daniela Jitaru2, B.M. Diaconescu3, Maria Cristina Vl\u0103deanu1, Iris Bararu-Bojan1, Carmen Anton4, Codru\u021ba B\u0103descu4<\/span><\/p>\n1Disciplina Fiziopatologie
\n2Institutul Regional de Oncologie Ia\u0219i
\n3Medicina muncii
\n4Departamentul de Medicin\u0103 intern\u0103
\nUniversitatea de Medicin\u0103 \u0219i Farmacie “Grigore T. Popa”, Ia\u015fi<\/p>\n
Cuvinte cheie: peptide antimicrobiene, melanom malign, defensin\u0103, catelicidin\u0103<\/p>\n
Introducere: Melanomul malign este cea mai grav\u0103 form\u0103 de cancer de piele. \u00cen ciuda progreselor realizate \u00een reducerea ratelor mortalit\u0103\u021bii cauzate de cancer din ultimii ani, dezvoltarea unor noi abord\u0103ri terapeutice r\u0103m\u00e2ne o prioritate.<\/p>\n
Material \u0219i metod\u0103: Plec\u00e2nd de la datele din literatur\u0103, conform c\u0103rora peptidele antimicrobiene (PAM) au poten\u021bial antitumoral, s-a evaluat in vitro poten\u021bialul tumoricid al defensinei beta1 \u0219i catelicidinei LL37 asupra unei linii celulare de melanom malign (A375) \u0219i a unei linii de celule epiteliale normale (HMLE). Ambele linii celulare au fost de provenien\u021b\u0103 uman\u0103.<\/p>\n
Rezultate: Am analizat efectele acestor PAM asupra viabilit\u0103\u021bii, citotoxicit\u0103\u021bii \u0219i prolifer\u0103rii melanocitelor tumorale \u0219i keratinocitelor normale. Studiul de viabilitate a fost realizat folosind anexin\u0103 \u0219i 7-AAD (7-amino-actinomicin\u0103 D), pentru detectarea \u0219i m\u0103surarea apoptozei prin tehnica citometriei \u00een flux. Rezultatele studiului nostru au relevat faptul c\u0103 citotoxicitatea catelicidinei LL37 \u0219i a defensinei a fost semnificativ\u0103 pentru linia de celule tumorale de tip melanom malign (A375) \u0219i nu a afectat semnificativ linia de celule epiteliale normale (HMLE), pentru care apoptoza celular\u0103 a fost de 4% la concentra\u021bii mici de LL37 \u0219i de 16% pentru defensin\u0103. La concentra\u021bii mari (15 \u00b5M) dup\u0103 o incubare de 48 h, apoptoza liniei HMLE a fost sub 30% at\u00e2t pentru defensin\u0103 c\u00e2t \u0219i pentru catelicidina LL37.<\/p>\n
Concluzii: PAM studiate pot deveni o alternativ\u0103 terapeutic\u0103 pentru melanomul malign, cu at\u00e2t mai mult cu c\u00e2t acestea nu afecteaz\u0103 integritatea celulelor epitaliale normale.<\/p>\n
Antimicrobial peptides a hope in melanoma therapy<\/span>
\nAutori: Magda B\u0103descu1, Manuela Ciocoiu1, Daniela Jitaru2, B.M. Diaconescu3, Maria Cristina Vl\u0103deanu1, Iris Bararu-Bojan1, Carmen Anton4, Codru\u021ba B\u0103descu4<\/span>
\n1Pathophysiology Department
\n2Regional Institute of Oncology Iasi
\n3Occupational medicine
\n4Internal Medicine Department
\nUniversity of Medicine and Pharmacy “Grigore T. Popa”, Ia\u015fi<\/p>\nKeywords: antimicrobial peptides, malignant melanoma, defensin, catelicidin<\/p>\n
Introduction: Malignant melanoma is the most serious form of skin cancer. Despite progress in reducing cancer mortality rates in recent years, the development of new therapeutic approaches remains a priority.<\/p>\n
Material and method: Based on literature data that antimicrobial peptides (MAPs) have antitumor potential, the tumoricidal potential of defensin beta1 and catelicidine LL37 was evaluated in vitro on a malignant melanoma cell line (A375) and a normal epithelial cell line (HMLE). ). Both cell lines were of human origin.<\/p>\n
Results: We analyzed the effects of these MAPs on the viability, cytotoxicity and proliferation of tumor melanocytes and normal keratinocytes. The feasibility study was performed using annexin and 7-AAD (7-amino-actinomycin D), to detect and measure apoptosis by flow cytometry. The results of our study revealed that the cytotoxicity of catelicidin LL37 and defensin was significant for the malignant melanoma tumor cell line (A375) and did not significantly affect the normal epithelial cell line (HMLE), for which cell apoptosis was 4 % at low concentrations of LL37 and 16% for defensin. At high concentrations (15 \u00b5M) after a 48 h incubation, HMLE line apoptosis was below 30% for both defensin and catelicidin LL37.<\/p>\n
Conclusions: The studied PAMs {defensin beta1 and catelicidine LL37) can become a therapeutic alternative for malignant melanoma, especially since they do not affect the integrity of normal epithelial cells.<\/p>\n
Scurt CV<\/span><\/p>\nProfesor emerit, Profesor Universitar Asociat Disciplina Fiziopatologie, Facultatea de Medicin\u0103, U.M.F. “Grigore T. Popa\u201d Ia\u015fi
\nConduc\u0103tor de doctorat
\nMedic primar medicin\u0103 intern\u0103
\nMembru a 15 societ\u0103\u0163i \u015ftiin\u0163ifice na\u0163ionale \u015fi interna\u0163ionale:
\nActivitatea \u015ftiin\u0163ific\u0103 se materializeaz\u0103 \u00een:
\n\u2022 16 manuale didactice \u015fi 6 monografii
\n\u2022 51 lucr\u0103ri \u00een reviste de specialitate cotate ISI in extenso
\n\u2022 131 lucr\u0103ri \u00een reviste de specialitate indexate BDI
\n\u2022 Factor de impact cumulat (pentru toate articolele) = 74,28
\n\u2022 C\u0103r\u0163i, capitole \u00een c\u0103r\u0163i publicate \u00een edituri na\u0163ionale: 53
\n\u2022 Capitole \u00een c\u0103r\u0163i publicate \u00een edituri interna\u0163ionale: 3
\n\u2022 Publica\u0163ii web: 159
\n\u2022 Index Hirsch: 9
\n\u2022 11 programe de cercetare din care director la 2 granturi
\n\u2022 24 teze de doctorat \u00eendrumate, finalizate \u0219i confirmate de CNATDCU<\/p>\n
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Contacteaz\u0103 operatorul PDI 2021<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@pdi.ro\/2021\/2019″ submit_button_text=”Trimite mesajul” _builder_version=”4.9.4″ title_font=”||||||||” custom_button=”on” button_text_color=”#F0622A”][et_pb_contact_field field_id=”Name” field_title=”Nume” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”Adresa de e-mail” field_type=”email” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Mesajul dvs.” field_type=”text” fullwidth_field=”on” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark”]<\/p>\n
Operatorul PDI 2021<\/p>\n
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![\"\"](\"https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2020\/02\/eventer_logo_150px.png\")
<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAdresa: Str. A. Panu nr. 13, Iasi<\/span>
Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off”]<\/p>\n
Parteneri Media<\/p>\n
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Prof. Dr. B\u0103descu Magda Mariana Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fiPeptidele antimicrobiene o speran\u021b\u0103 \u00een terapia melanomului malign Autori: Magda B\u0103descu1, Manuela Ciocoiu1, Daniela Jitaru2, B.M. Diaconescu3, Maria Cristina Vl\u0103deanu1, Iris Bararu-Bojan1, Carmen Anton4, Codru\u021ba B\u0103descu4 1Disciplina Fiziopatologie 2Institutul Regional de Oncologie Ia\u0219i 3Medicina muncii 4Departamentul de Medicin\u0103 intern\u0103 Universitatea de Medicin\u0103 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-7478","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7478","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/comments?post=7478"}],"version-history":[{"count":9,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7478\/revisions"}],"predecessor-version":[{"id":9576,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7478\/revisions\/9576"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/media?parent=7478"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/strong><\/span><\/h5>\n<\/strong><\/span><\/h5>\nProf. Dr. B\u0103descu Magda Mariana<\/strong><\/span>
Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fi<\/strong><\/span><\/h5>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ locked=”off”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.6″]<\/p>\n
Peptidele antimicrobiene o speran\u021b\u0103 \u00een terapia melanomului malign<\/span>
\n Autori: Magda B\u0103descu1, Manuela Ciocoiu1, Daniela Jitaru2, B.M. Diaconescu3, Maria Cristina Vl\u0103deanu1, Iris Bararu-Bojan1, Carmen Anton4, Codru\u021ba B\u0103descu4<\/span><\/p>\n1Disciplina Fiziopatologie
\n2Institutul Regional de Oncologie Ia\u0219i
\n3Medicina muncii
\n4Departamentul de Medicin\u0103 intern\u0103
\nUniversitatea de Medicin\u0103 \u0219i Farmacie “Grigore T. Popa”, Ia\u015fi<\/p>\n
Cuvinte cheie: peptide antimicrobiene, melanom malign, defensin\u0103, catelicidin\u0103<\/p>\n
Introducere: Melanomul malign este cea mai grav\u0103 form\u0103 de cancer de piele. \u00cen ciuda progreselor realizate \u00een reducerea ratelor mortalit\u0103\u021bii cauzate de cancer din ultimii ani, dezvoltarea unor noi abord\u0103ri terapeutice r\u0103m\u00e2ne o prioritate.<\/p>\n
Material \u0219i metod\u0103: Plec\u00e2nd de la datele din literatur\u0103, conform c\u0103rora peptidele antimicrobiene (PAM) au poten\u021bial antitumoral, s-a evaluat in vitro poten\u021bialul tumoricid al defensinei beta1 \u0219i catelicidinei LL37 asupra unei linii celulare de melanom malign (A375) \u0219i a unei linii de celule epiteliale normale (HMLE). Ambele linii celulare au fost de provenien\u021b\u0103 uman\u0103.<\/p>\n
Rezultate: Am analizat efectele acestor PAM asupra viabilit\u0103\u021bii, citotoxicit\u0103\u021bii \u0219i prolifer\u0103rii melanocitelor tumorale \u0219i keratinocitelor normale. Studiul de viabilitate a fost realizat folosind anexin\u0103 \u0219i 7-AAD (7-amino-actinomicin\u0103 D), pentru detectarea \u0219i m\u0103surarea apoptozei prin tehnica citometriei \u00een flux. Rezultatele studiului nostru au relevat faptul c\u0103 citotoxicitatea catelicidinei LL37 \u0219i a defensinei a fost semnificativ\u0103 pentru linia de celule tumorale de tip melanom malign (A375) \u0219i nu a afectat semnificativ linia de celule epiteliale normale (HMLE), pentru care apoptoza celular\u0103 a fost de 4% la concentra\u021bii mici de LL37 \u0219i de 16% pentru defensin\u0103. La concentra\u021bii mari (15 \u00b5M) dup\u0103 o incubare de 48 h, apoptoza liniei HMLE a fost sub 30% at\u00e2t pentru defensin\u0103 c\u00e2t \u0219i pentru catelicidina LL37.<\/p>\n
Concluzii: PAM studiate pot deveni o alternativ\u0103 terapeutic\u0103 pentru melanomul malign, cu at\u00e2t mai mult cu c\u00e2t acestea nu afecteaz\u0103 integritatea celulelor epitaliale normale.<\/p>\n
Antimicrobial peptides a hope in melanoma therapy<\/span>
\nAutori: Magda B\u0103descu1, Manuela Ciocoiu1, Daniela Jitaru2, B.M. Diaconescu3, Maria Cristina Vl\u0103deanu1, Iris Bararu-Bojan1, Carmen Anton4, Codru\u021ba B\u0103descu4<\/span>
\n1Pathophysiology Department
\n2Regional Institute of Oncology Iasi
\n3Occupational medicine
\n4Internal Medicine Department
\nUniversity of Medicine and Pharmacy “Grigore T. Popa”, Ia\u015fi<\/p>\nKeywords: antimicrobial peptides, malignant melanoma, defensin, catelicidin<\/p>\n
Introduction: Malignant melanoma is the most serious form of skin cancer. Despite progress in reducing cancer mortality rates in recent years, the development of new therapeutic approaches remains a priority.<\/p>\n
Material and method: Based on literature data that antimicrobial peptides (MAPs) have antitumor potential, the tumoricidal potential of defensin beta1 and catelicidine LL37 was evaluated in vitro on a malignant melanoma cell line (A375) and a normal epithelial cell line (HMLE). ). Both cell lines were of human origin.<\/p>\n
Results: We analyzed the effects of these MAPs on the viability, cytotoxicity and proliferation of tumor melanocytes and normal keratinocytes. The feasibility study was performed using annexin and 7-AAD (7-amino-actinomycin D), to detect and measure apoptosis by flow cytometry. The results of our study revealed that the cytotoxicity of catelicidin LL37 and defensin was significant for the malignant melanoma tumor cell line (A375) and did not significantly affect the normal epithelial cell line (HMLE), for which cell apoptosis was 4 % at low concentrations of LL37 and 16% for defensin. At high concentrations (15 \u00b5M) after a 48 h incubation, HMLE line apoptosis was below 30% for both defensin and catelicidin LL37.<\/p>\n
Conclusions: The studied PAMs {defensin beta1 and catelicidine LL37) can become a therapeutic alternative for malignant melanoma, especially since they do not affect the integrity of normal epithelial cells.<\/p>\n
Scurt CV<\/span><\/p>\nProfesor emerit, Profesor Universitar Asociat Disciplina Fiziopatologie, Facultatea de Medicin\u0103, U.M.F. “Grigore T. Popa\u201d Ia\u015fi
\nConduc\u0103tor de doctorat
\nMedic primar medicin\u0103 intern\u0103
\nMembru a 15 societ\u0103\u0163i \u015ftiin\u0163ifice na\u0163ionale \u015fi interna\u0163ionale:
\nActivitatea \u015ftiin\u0163ific\u0103 se materializeaz\u0103 \u00een:
\n\u2022 16 manuale didactice \u015fi 6 monografii
\n\u2022 51 lucr\u0103ri \u00een reviste de specialitate cotate ISI in extenso
\n\u2022 131 lucr\u0103ri \u00een reviste de specialitate indexate BDI
\n\u2022 Factor de impact cumulat (pentru toate articolele) = 74,28
\n\u2022 C\u0103r\u0163i, capitole \u00een c\u0103r\u0163i publicate \u00een edituri na\u0163ionale: 53
\n\u2022 Capitole \u00een c\u0103r\u0163i publicate \u00een edituri interna\u0163ionale: 3
\n\u2022 Publica\u0163ii web: 159
\n\u2022 Index Hirsch: 9
\n\u2022 11 programe de cercetare din care director la 2 granturi
\n\u2022 24 teze de doctorat \u00eendrumate, finalizate \u0219i confirmate de CNATDCU<\/p>\n
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Contacteaz\u0103 operatorul PDI 2021<\/p>\n
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Operatorul PDI 2021<\/p>\n
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<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAdresa: Str. A. Panu nr. 13, Iasi<\/span>
Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off”]<\/p>\n
Parteneri Media<\/p>\n
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Prof. Dr. B\u0103descu Magda Mariana Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fiPeptidele antimicrobiene o speran\u021b\u0103 \u00een terapia melanomului malign Autori: Magda B\u0103descu1, Manuela Ciocoiu1, Daniela Jitaru2, B.M. Diaconescu3, Maria Cristina Vl\u0103deanu1, Iris Bararu-Bojan1, Carmen Anton4, Codru\u021ba B\u0103descu4 1Disciplina Fiziopatologie 2Institutul Regional de Oncologie Ia\u0219i 3Medicina muncii 4Departamentul de Medicin\u0103 intern\u0103 Universitatea de Medicin\u0103 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-7478","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7478","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/comments?post=7478"}],"version-history":[{"count":9,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7478\/revisions"}],"predecessor-version":[{"id":9576,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7478\/revisions\/9576"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/media?parent=7478"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Prof. Dr. B\u0103descu Magda Mariana<\/strong><\/span>
Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fi<\/strong><\/span><\/h5>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ locked=”off”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.6″]<\/p>\n
Peptidele antimicrobiene o speran\u021b\u0103 \u00een terapia melanomului malign<\/span>
\n Autori: Magda B\u0103descu1, Manuela Ciocoiu1, Daniela Jitaru2, B.M. Diaconescu3, Maria Cristina Vl\u0103deanu1, Iris Bararu-Bojan1, Carmen Anton4, Codru\u021ba B\u0103descu4<\/span><\/p>\n1Disciplina Fiziopatologie
\n2Institutul Regional de Oncologie Ia\u0219i
\n3Medicina muncii
\n4Departamentul de Medicin\u0103 intern\u0103
\nUniversitatea de Medicin\u0103 \u0219i Farmacie “Grigore T. Popa”, Ia\u015fi<\/p>\n
Cuvinte cheie: peptide antimicrobiene, melanom malign, defensin\u0103, catelicidin\u0103<\/p>\n
Introducere: Melanomul malign este cea mai grav\u0103 form\u0103 de cancer de piele. \u00cen ciuda progreselor realizate \u00een reducerea ratelor mortalit\u0103\u021bii cauzate de cancer din ultimii ani, dezvoltarea unor noi abord\u0103ri terapeutice r\u0103m\u00e2ne o prioritate.<\/p>\n
Material \u0219i metod\u0103: Plec\u00e2nd de la datele din literatur\u0103, conform c\u0103rora peptidele antimicrobiene (PAM) au poten\u021bial antitumoral, s-a evaluat in vitro poten\u021bialul tumoricid al defensinei beta1 \u0219i catelicidinei LL37 asupra unei linii celulare de melanom malign (A375) \u0219i a unei linii de celule epiteliale normale (HMLE). Ambele linii celulare au fost de provenien\u021b\u0103 uman\u0103.<\/p>\n
Rezultate: Am analizat efectele acestor PAM asupra viabilit\u0103\u021bii, citotoxicit\u0103\u021bii \u0219i prolifer\u0103rii melanocitelor tumorale \u0219i keratinocitelor normale. Studiul de viabilitate a fost realizat folosind anexin\u0103 \u0219i 7-AAD (7-amino-actinomicin\u0103 D), pentru detectarea \u0219i m\u0103surarea apoptozei prin tehnica citometriei \u00een flux. Rezultatele studiului nostru au relevat faptul c\u0103 citotoxicitatea catelicidinei LL37 \u0219i a defensinei a fost semnificativ\u0103 pentru linia de celule tumorale de tip melanom malign (A375) \u0219i nu a afectat semnificativ linia de celule epiteliale normale (HMLE), pentru care apoptoza celular\u0103 a fost de 4% la concentra\u021bii mici de LL37 \u0219i de 16% pentru defensin\u0103. La concentra\u021bii mari (15 \u00b5M) dup\u0103 o incubare de 48 h, apoptoza liniei HMLE a fost sub 30% at\u00e2t pentru defensin\u0103 c\u00e2t \u0219i pentru catelicidina LL37.<\/p>\n
Concluzii: PAM studiate pot deveni o alternativ\u0103 terapeutic\u0103 pentru melanomul malign, cu at\u00e2t mai mult cu c\u00e2t acestea nu afecteaz\u0103 integritatea celulelor epitaliale normale.<\/p>\n
Antimicrobial peptides a hope in melanoma therapy<\/span>
\nAutori: Magda B\u0103descu1, Manuela Ciocoiu1, Daniela Jitaru2, B.M. Diaconescu3, Maria Cristina Vl\u0103deanu1, Iris Bararu-Bojan1, Carmen Anton4, Codru\u021ba B\u0103descu4<\/span>
\n1Pathophysiology Department
\n2Regional Institute of Oncology Iasi
\n3Occupational medicine
\n4Internal Medicine Department
\nUniversity of Medicine and Pharmacy “Grigore T. Popa”, Ia\u015fi<\/p>\nKeywords: antimicrobial peptides, malignant melanoma, defensin, catelicidin<\/p>\n
Introduction: Malignant melanoma is the most serious form of skin cancer. Despite progress in reducing cancer mortality rates in recent years, the development of new therapeutic approaches remains a priority.<\/p>\n
Material and method: Based on literature data that antimicrobial peptides (MAPs) have antitumor potential, the tumoricidal potential of defensin beta1 and catelicidine LL37 was evaluated in vitro on a malignant melanoma cell line (A375) and a normal epithelial cell line (HMLE). ). Both cell lines were of human origin.<\/p>\n
Results: We analyzed the effects of these MAPs on the viability, cytotoxicity and proliferation of tumor melanocytes and normal keratinocytes. The feasibility study was performed using annexin and 7-AAD (7-amino-actinomycin D), to detect and measure apoptosis by flow cytometry. The results of our study revealed that the cytotoxicity of catelicidin LL37 and defensin was significant for the malignant melanoma tumor cell line (A375) and did not significantly affect the normal epithelial cell line (HMLE), for which cell apoptosis was 4 % at low concentrations of LL37 and 16% for defensin. At high concentrations (15 \u00b5M) after a 48 h incubation, HMLE line apoptosis was below 30% for both defensin and catelicidin LL37.<\/p>\n
Conclusions: The studied PAMs {defensin beta1 and catelicidine LL37) can become a therapeutic alternative for malignant melanoma, especially since they do not affect the integrity of normal epithelial cells.<\/p>\n
Scurt CV<\/span><\/p>\nProfesor emerit, Profesor Universitar Asociat Disciplina Fiziopatologie, Facultatea de Medicin\u0103, U.M.F. “Grigore T. Popa\u201d Ia\u015fi
\nConduc\u0103tor de doctorat
\nMedic primar medicin\u0103 intern\u0103
\nMembru a 15 societ\u0103\u0163i \u015ftiin\u0163ifice na\u0163ionale \u015fi interna\u0163ionale:
\nActivitatea \u015ftiin\u0163ific\u0103 se materializeaz\u0103 \u00een:
\n\u2022 16 manuale didactice \u015fi 6 monografii
\n\u2022 51 lucr\u0103ri \u00een reviste de specialitate cotate ISI in extenso
\n\u2022 131 lucr\u0103ri \u00een reviste de specialitate indexate BDI
\n\u2022 Factor de impact cumulat (pentru toate articolele) = 74,28
\n\u2022 C\u0103r\u0163i, capitole \u00een c\u0103r\u0163i publicate \u00een edituri na\u0163ionale: 53
\n\u2022 Capitole \u00een c\u0103r\u0163i publicate \u00een edituri interna\u0163ionale: 3
\n\u2022 Publica\u0163ii web: 159
\n\u2022 Index Hirsch: 9
\n\u2022 11 programe de cercetare din care director la 2 granturi
\n\u2022 24 teze de doctorat \u00eendrumate, finalizate \u0219i confirmate de CNATDCU<\/p>\n
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Contacteaz\u0103 operatorul PDI 2021<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@pdi.ro\/2021\/2019″ submit_button_text=”Trimite mesajul” _builder_version=”4.9.4″ title_font=”||||||||” custom_button=”on” button_text_color=”#F0622A”][et_pb_contact_field field_id=”Name” field_title=”Nume” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”Adresa de e-mail” field_type=”email” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Mesajul dvs.” field_type=”text” fullwidth_field=”on” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark”]<\/p>\n
Operatorul PDI 2021<\/p>\n
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<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAdresa: Str. A. Panu nr. 13, Iasi<\/span>
Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off”]<\/p>\n
Parteneri Media<\/p>\n
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Prof. Dr. B\u0103descu Magda Mariana Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fiPeptidele antimicrobiene o speran\u021b\u0103 \u00een terapia melanomului malign Autori: Magda B\u0103descu1, Manuela Ciocoiu1, Daniela Jitaru2, B.M. Diaconescu3, Maria Cristina Vl\u0103deanu1, Iris Bararu-Bojan1, Carmen Anton4, Codru\u021ba B\u0103descu4 1Disciplina Fiziopatologie 2Institutul Regional de Oncologie Ia\u0219i 3Medicina muncii 4Departamentul de Medicin\u0103 intern\u0103 Universitatea de Medicin\u0103 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-7478","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7478","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/comments?post=7478"}],"version-history":[{"count":9,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7478\/revisions"}],"predecessor-version":[{"id":9576,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7478\/revisions\/9576"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/media?parent=7478"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\n Autori: Magda B\u0103descu1, Manuela Ciocoiu1, Daniela Jitaru2, B.M. Diaconescu3, Maria Cristina Vl\u0103deanu1, Iris Bararu-Bojan1, Carmen Anton4, Codru\u021ba B\u0103descu4<\/span><\/p>\n
1Disciplina Fiziopatologie
\n2Institutul Regional de Oncologie Ia\u0219i
\n3Medicina muncii
\n4Departamentul de Medicin\u0103 intern\u0103
\nUniversitatea de Medicin\u0103 \u0219i Farmacie “Grigore T. Popa”, Ia\u015fi<\/p>\n
Cuvinte cheie: peptide antimicrobiene, melanom malign, defensin\u0103, catelicidin\u0103<\/p>\n
Introducere: Melanomul malign este cea mai grav\u0103 form\u0103 de cancer de piele. \u00cen ciuda progreselor realizate \u00een reducerea ratelor mortalit\u0103\u021bii cauzate de cancer din ultimii ani, dezvoltarea unor noi abord\u0103ri terapeutice r\u0103m\u00e2ne o prioritate.<\/p>\n
Material \u0219i metod\u0103: Plec\u00e2nd de la datele din literatur\u0103, conform c\u0103rora peptidele antimicrobiene (PAM) au poten\u021bial antitumoral, s-a evaluat in vitro poten\u021bialul tumoricid al defensinei beta1 \u0219i catelicidinei LL37 asupra unei linii celulare de melanom malign (A375) \u0219i a unei linii de celule epiteliale normale (HMLE). Ambele linii celulare au fost de provenien\u021b\u0103 uman\u0103.<\/p>\n
Rezultate: Am analizat efectele acestor PAM asupra viabilit\u0103\u021bii, citotoxicit\u0103\u021bii \u0219i prolifer\u0103rii melanocitelor tumorale \u0219i keratinocitelor normale. Studiul de viabilitate a fost realizat folosind anexin\u0103 \u0219i 7-AAD (7-amino-actinomicin\u0103 D), pentru detectarea \u0219i m\u0103surarea apoptozei prin tehnica citometriei \u00een flux. Rezultatele studiului nostru au relevat faptul c\u0103 citotoxicitatea catelicidinei LL37 \u0219i a defensinei a fost semnificativ\u0103 pentru linia de celule tumorale de tip melanom malign (A375) \u0219i nu a afectat semnificativ linia de celule epiteliale normale (HMLE), pentru care apoptoza celular\u0103 a fost de 4% la concentra\u021bii mici de LL37 \u0219i de 16% pentru defensin\u0103. La concentra\u021bii mari (15 \u00b5M) dup\u0103 o incubare de 48 h, apoptoza liniei HMLE a fost sub 30% at\u00e2t pentru defensin\u0103 c\u00e2t \u0219i pentru catelicidina LL37.<\/p>\n
Concluzii: PAM studiate pot deveni o alternativ\u0103 terapeutic\u0103 pentru melanomul malign, cu at\u00e2t mai mult cu c\u00e2t acestea nu afecteaz\u0103 integritatea celulelor epitaliale normale.<\/p>\n
Antimicrobial peptides a hope in melanoma therapy<\/span> Keywords: antimicrobial peptides, malignant melanoma, defensin, catelicidin<\/p>\n Introduction: Malignant melanoma is the most serious form of skin cancer. Despite progress in reducing cancer mortality rates in recent years, the development of new therapeutic approaches remains a priority.<\/p>\n Material and method: Based on literature data that antimicrobial peptides (MAPs) have antitumor potential, the tumoricidal potential of defensin beta1 and catelicidine LL37 was evaluated in vitro on a malignant melanoma cell line (A375) and a normal epithelial cell line (HMLE). ). Both cell lines were of human origin.<\/p>\n Results: We analyzed the effects of these MAPs on the viability, cytotoxicity and proliferation of tumor melanocytes and normal keratinocytes. The feasibility study was performed using annexin and 7-AAD (7-amino-actinomycin D), to detect and measure apoptosis by flow cytometry. The results of our study revealed that the cytotoxicity of catelicidin LL37 and defensin was significant for the malignant melanoma tumor cell line (A375) and did not significantly affect the normal epithelial cell line (HMLE), for which cell apoptosis was 4 % at low concentrations of LL37 and 16% for defensin. At high concentrations (15 \u00b5M) after a 48 h incubation, HMLE line apoptosis was below 30% for both defensin and catelicidin LL37.<\/p>\n Conclusions: The studied PAMs {defensin beta1 and catelicidine LL37) can become a therapeutic alternative for malignant melanoma, especially since they do not affect the integrity of normal epithelial cells.<\/p>\n Scurt CV<\/span><\/p>\n Profesor emerit, Profesor Universitar Asociat Disciplina Fiziopatologie, Facultatea de Medicin\u0103, U.M.F. “Grigore T. 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Diaconescu3, Maria Cristina Vl\u0103deanu1, Iris Bararu-Bojan1, Carmen Anton4, Codru\u021ba B\u0103descu4 1Disciplina Fiziopatologie 2Institutul Regional de Oncologie Ia\u0219i 3Medicina muncii 4Departamentul de Medicin\u0103 intern\u0103 Universitatea de Medicin\u0103 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-7478","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7478","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/comments?post=7478"}],"version-history":[{"count":9,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7478\/revisions"}],"predecessor-version":[{"id":9576,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7478\/revisions\/9576"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/media?parent=7478"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nAutori: Magda B\u0103descu1, Manuela Ciocoiu1, Daniela Jitaru2, B.M. Diaconescu3, Maria Cristina Vl\u0103deanu1, Iris Bararu-Bojan1, Carmen Anton4, Codru\u021ba B\u0103descu4<\/span>
\n1Pathophysiology Department
\n2Regional Institute of Oncology Iasi
\n3Occupational medicine
\n4Internal Medicine Department
\nUniversity of Medicine and Pharmacy “Grigore T. Popa”, Ia\u015fi<\/p>\n
\nConduc\u0103tor de doctorat
\nMedic primar medicin\u0103 intern\u0103
\nMembru a 15 societ\u0103\u0163i \u015ftiin\u0163ifice na\u0163ionale \u015fi interna\u0163ionale:
\nActivitatea \u015ftiin\u0163ific\u0103 se materializeaz\u0103 \u00een:
\n\u2022 16 manuale didactice \u015fi 6 monografii
\n\u2022 51 lucr\u0103ri \u00een reviste de specialitate cotate ISI in extenso
\n\u2022 131 lucr\u0103ri \u00een reviste de specialitate indexate BDI
\n\u2022 Factor de impact cumulat (pentru toate articolele) = 74,28
\n\u2022 C\u0103r\u0163i, capitole \u00een c\u0103r\u0163i publicate \u00een edituri na\u0163ionale: 53
\n\u2022 Capitole \u00een c\u0103r\u0163i publicate \u00een edituri interna\u0163ionale: 3
\n\u2022 Publica\u0163ii web: 159
\n\u2022 Index Hirsch: 9
\n\u2022 11 programe de cercetare din care director la 2 granturi
\n\u2022 24 teze de doctorat \u00eendrumate, finalizate \u0219i confirmate de CNATDCU<\/p>\n<\/span><\/p>\n
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