{"id":7646,"date":"2020-03-06T17:53:14","date_gmt":"2020-03-06T15:53:14","guid":{"rendered":"https:\/\/primaderma.ro\/?page_id=7646"},"modified":"2021-07-02T15:15:30","modified_gmt":"2021-07-02T13:15:30","slug":"cercetator-senior-carolina-constantin","status":"publish","type":"page","link":"https:\/\/pdi.ro\/2021\/cercetator-senior-carolina-constantin\/","title":{"rendered":"Cercet\u0103tor Senior Constantin Carolina"},"content":{"rendered":"

[et_pb_section fb_built=”1″ fullwidth=”on” disabled_on=”on|off|off” _builder_version=”3.22″ custom_margin=”||” custom_padding=”15px||29px” top_divider_style=”waves2″ top_divider_color=”#f0622a” top_divider_flip=”horizontal|vertical” bottom_divider_style=”waves2″ bottom_divider_color=”#fcdedb” bottom_divider_height=”105px” bottom_divider_flip=”vertical” collapsed=”off” global_module=”9062″][et_pb_fullwidth_header title=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF90aXRsZSIsInNldHRpbmdzIjp7ImJlZm9yZSI6IiIsImFmdGVyIjoiIn19@” text_orientation=”right” header_image_url=”https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2021\/05\/PDI2021_logo_header_int_600x300px.png” disabled_on=”on|on|off” _builder_version=”4.9.4″ _dynamic_attributes=”title” title_font=”Kaushan Script||||||||” title_text_align=”right” title_text_color=”#f0622a” title_font_size=”50px” content_font=”|600||on|||||” content_text_align=”center” content_text_color=”#686766″ content_font_size=”15px” subhead_font=”||||||||” background_color=”#ef916b” background_image=”https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2021\/05\/PDI2021_header_1920x550px.jpg” background_blend=”luminosity” background_layout=”light” custom_margin=”0px||8px” custom_padding=”40px||40px||true” content_font_size_tablet=”” content_font_size_phone=”15px” content_font_size_last_edited=”on|desktop”][\/et_pb_fullwidth_header][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”3.22″][et_pb_row column_structure=”2_5,3_5″ _builder_version=”3.25″][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2020\/03\/Constantin-Carolina.jpg” align=”center” align_tablet=”center” align_phone=”” align_last_edited=”on|desktop” _builder_version=”4.4.0″ max_width=”30%” border_radii=”on|100px|100px|100px|100px”][\/et_pb_image][\/et_pb_column][et_pb_column type=”3_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.6″]<\/p>\n

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Cercet\u0103tor Senior Constantin Carolina <\/span><\/strong>
\n<\/span><\/strong><\/h4>\n

Institutul Na\u0163ional de Cercetare-Dezvoltare \u00een Domeniul Patologiei \u015fi \u015etiin\u0163elor Biomedicale “Victor Babe\u015f\u201d Bucure\u015fti
\nServiciul de Anatomie Patologic\u0103, Spitalul Clinic Colentina, Bucure\u015fti<\/span><\/strong><\/p>\n

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Perspective epigenomice ca surs\u0103 de biomarkeri \u00een tumorile cutanate
Autori: Carolina Constantin1,2, Mihaela Surcel1, Adriana Narcisa Munteanu1,3, Ana C\u0103runtu4, C. C\u0103runtu5, Sabina Andrada Zurac2, 6, Monica Neagu1,2,3<\/span>
1Sec\u021bia Imunologie, Institutul Na\u0163ional de Cercetare-Dezvoltare \u00een Domeniul Patologiei \u015fi \u015etiin\u0163elor Biomedicale “Victor Babe\u015f\u201d Bucure\u015fti
2Serviciul de Anatomie Patologic\u0103, Spitalul Clinic Colentina, Bucure\u015fti
3\u0218coala Doctoral\u0103, Facultatea de Biologie, Universitatea Bucure\u015fti
4Departmentul de Chirurgie Oral\u0103 \u0219i Maxilofacial\u0103, Spitalul de Urgen\u021b\u0103 Militar Central \u201cDr. Carol Davila\u201d, Bucure\u015fti
5Departamentul de Fiziologie, Universitatea de Medicin\u0103 \u015fi Farmacie “Carol Davila\u201d, Bucure\u015fti
6Departamentul de Patologie, Facultatea de Medicin\u0103 Dentar\u0103, Universitatea de Medicin\u0103 \u015fi Farmacie “Carol Davila\u201d, Bucure\u015fti<\/p>\n

Cuvinte cheie: biomarker, epigenom, metilare, tumori piele<\/p>\n

Introducere: Epigenomul include o componenta relativ recent\u0103 \u2013 epitranscriptomul, ce define\u0219te modific\u0103rile suferite de ARN, \u0219i care se alatur\u0103 epigeneticii \u00een l\u0103rgirea arsenalului de markeri \u00een contextul tumoral. Majoritatea studiilor legate de epigenetica proceselor tumorale se focuseaz\u0103 pe c\u0103ile oncogene tumorale la nivelul tumorii primare, astfel \u00eenc\u00e2t efectele \u0219i rolul modific\u0103rii de tipul N6-metiladenozina (m6A) \u00een ARN-ul din celulele s\u00e2ngelui periferic, sunt extrem de pu\u021bin abordate, fiind \u00een mare parte necunoscute. \u00cen tumorile cutanate, studiile privind valorile poten\u021biale de diagnostic \u0219i prognostic ale leucocitelor periferice prin prisma modific\u0103rilor epitranscriptomice m6A pot furniza noi markeri pentru managementul bolii.
Material \u0219i metod\u0103: Celule mononucleare (PBMC) au fost izolate din sange periferic de la subiecti cu carcinom bazocelular (BCC), spinocelular (SCC) sau melanom cutanat. Din suspensiile celulare s-a extras ARN total la care s-a evaluat gradul de metilare m6A printr-o platform\u0103 de tip ELISA bazat\u0103 pe anticorpi specifici anti-m6A.
Rezultate: Indicele c\u00e2t \u0219i procentul de metilare m6A la nivelul ARN din leucocite PBMC este crescut la tumorile de piele comparativ cu normal. De asemenea, exist\u0103 diferen\u021be \u00een nivelul de metilare la tumori non-melanom versus melanom, \u00een cazul melanomului cutanat indicele m6A fiind dublu fa\u021b\u0103 de SCC \u0219i de cel putin 10x mai ridicat comparativ cu BCC.
Concluzii: Nivelul m6A poate conferi identitate transcriptomic\u0103 \u00een leucocitele periferie \u0219i poate constitui un nou biomarker de grani\u021b\u0103 epi-imun, neinvaziv, cu utilitate poten\u021bial\u0103 at\u00e2t \u00een discriminarea subtipurilor de tumori cutanate dar \u0219i \u00een monitorizarea clinic\u0103 a pacientului.
Studiu finan\u021bat prin proiectele: PN-III-P1-1.2-PCCDI-2017-0341\/2018, PN 19.29.01.01\/2019, EPITRAN COST ACTION 16120\/2018.<\/p>\n

Epigenomic perspectives as biomarkers source in skin cancers
Autori: Carolina Constantin1,2, Mihaela Surcel1, Adriana Narcisa Munteanu1,3, Ana C\u0103runtu4, C. C\u0103runtu5, Sabina Andrada Zurac 2, 6, Monica Neagu 1, 2, 3 <\/span>
1Immunology Department, \u201eVictor Babes\u201d National Institute of Pathology, Bucharest
2Pathology Department, \u201eColentina\u201d University Hospital, Bucharest
3Doctoral School, Faculty of Biology, University of Bucharest, Bucharest
4Department of Oral and Maxillofacial Surgery, \u201cDr. Carol Davila\u201d Central Military Emergency Hospital, Bucharest
5Department of Physiology, \u201cCarol Davila\u201d University of Medicine and Pharmacy, Bucharest
6Pathology Department, Faculty of Dental Medicine, \u201cCarol Davila\u201d University of Medicine and Pharmacy, Bucharest<\/p>\n

Keywords: biomarker, epigenom, methylation, skin tumors<\/p>\n

Introduction. The epigenome includes a relatively recent component – the epitranscriptome, which defines the changes in RNA, and joins epigenetics in expanding the arsenal of tumor markers. Most studies related to the epigenetics of tumor processes focus on tumor oncogenic pathways in the primary tumor, so the effects and role of N6-methyladenosine (m6A) modification in RNA in peripheral blood cells are extremely poorly addressed. In skin tumors, studies on the potential diagnostic and prognostic values of peripheral leukocytes in the light of m6A epitranscriptomic changes may provide new markers for disease management.
Material and method. Mononuclear cells (PBMC) were isolated from peripheral blood from subjects with basal cell carcinoma (BCC), squamous cell carcinoma (SCC) or cutaneous melanoma. From the cell suspensions, total RNA was extracted and the m6A methylation level was assessed by an ELISA-type platform based on specific anti-m6A antibodies.
Results. The index and the percentage of m6A methylation in RNA from PBMC leukocytes is increased in skin tumors compared to normal. There are also differences in the level of methylation in non-melanoma versus melanoma, in the case of cutaneous melanoma the m6A index is double that of SCC and at least ten fold higher compared to BCC.
Conclusions. The m6A level can confer transcriptomic identity in the peripheral leukocytes and can be a new biomarker of epi-immune border, non-invasive, with potential utility both in discriminating skin tumor subtypes and in clinical monitoring of the patient.
Study financed by the following projects: PN-III-P1-1.2-PCCDI-2017-0341\/2018, PN 19.29.01.01\/2019, EPITRAN COST ACTION 16120\/2018.<\/p>\n

Scurt CV<\/span><\/p>\n

Senior researcher I, PhD, biochemist, \u201cVictor Babe\u0219\u201d National Institute of Pathology, Immunology Laboratory & Colentina Clinical Hospital, Research Core of Pathology Department, Bucharest.
Research topics: immune parameters analysis and biomarker discovery in melanoma and non-melanoma skin cancer; co-inventor for 4 patents related to photodynamic therapy PDT in skin pathologies. Dr. Constantin conducted national research projects related to novel photosensitizers for PDT in cancer, and drug-delivery nanosystems for skin regeneration; partner responsible for MNT_ERANET project 7030-1\/2010 related to novel porphyrins in theranostics. Specialization in molecular biology at Ludwig Institute for Cancer Research, Signal Transduction Unit (Bruxelles); cellular biology specialization at University of Athens, Biochemistry and Physiology Department, in the frame of NATO SfP 982838\/2007 project. Technical expert in 5 EU projects for biomedical assays; teaching experience in protein microarray technology; volunteer reviewer for several ISI journals.
Currently involved as MC member for COST Action CA18127 – International Nucleome Consortium (INC); MC substitute of the management committee for COST action CA16120 – European Epitranscriptomics Network (EPITRAN); alternate member of the management committee for CA18125 – Advanced Engineering and Research of Aerogels for Environment and Life Sciences (AEROGELS).<\/p>\n

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Contacteaz\u0103 operatorul PDI 2021<\/p>\n

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Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
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Cercet\u0103tor Senior Constantin Carolina Institutul Na\u0163ional de Cercetare-Dezvoltare \u00een Domeniul Patologiei \u015fi \u015etiin\u0163elor Biomedicale “Victor Babe\u015f\u201d Bucure\u015fti Serviciul de Anatomie Patologic\u0103, Spitalul Clinic Colentina, Bucure\u015ftiPerspective epigenomice ca surs\u0103 de biomarkeri \u00een tumorile cutanateAutori: Carolina Constantin1,2, Mihaela Surcel1, Adriana Narcisa Munteanu1,3, Ana C\u0103runtu4, C. C\u0103runtu5, Sabina Andrada Zurac2, 6, Monica Neagu1,2,31Sec\u021bia Imunologie, Institutul Na\u0163ional de Cercetare-Dezvoltare […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-7646","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7646","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/comments?post=7646"}],"version-history":[{"count":14,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7646\/revisions"}],"predecessor-version":[{"id":9687,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/7646\/revisions\/9687"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/media?parent=7646"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}