<\/span><\/strong><\/h4>\n<\/span><\/strong><\/h4>\nProf. Univ. Dr. Iulia Popescu<\/span><\/strong>
Allergy and Critical Care Division, Department Medicine, University of Pittsburgh, Pittsburgh, PA<\/span><\/strong><\/h4>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.6″]<\/p>\n
CD4+ T cell lymphopenia and dysfunction in severe COVID-19 disease is autocrine TNF-\u03b1\/TNFRI-dependent <\/span><\/strong>
\nAutori: Iulia Popescu, John F. McDyer<\/span><\/strong><\/p>\nOne Sentence Summary: Autocrine TNF-\u03b1\/TNFRI regulates CD4+ T cell lymphopenia and dysfunction in severe COVID-19 disease.<\/span><\/p>\nLymphopenia is common in severe COVID-19 disease, yet the mechanisms are poorly understood. In 148 patients with severe COVID-19, we found lymphopenia was associated with worse survival. CD4+ lymphopenia predominated, with lower CD4+\/CD8+ ratios in severe COVID-19 compared to recovered, mild disease (p<0.0001). In severe disease, immunodominant CD4+ T cell responses to Spike-1(S1) produced increased in vitro TNF-\u03b1, but impaired proliferation and increased susceptibility to activation-induced cell death (AICD). CD4+TNF-\u03b1+ T cell responses inversely correlated with absolute CD4+ counts from severe COVID-19 patients (n=76; R=-0.744, P<0.0001). TNF-\u03b1 blockade including infliximab or anti-TNFRI antibodies strikingly rescued S1-specific CD4+ proliferation and abrogated S1-AICD in severe COVID-19 patients (P<0.001). Single-cell RNAseq demonstrated downregulation of Type-1 cytokines and NFkB signaling in S1-stimulated CD4+ cells with infliximab treatment. Lung CD4+ T cells in severe COVID-19 were reduced and produced higher TNF-\u03b1 versus PBMC. Together, our findings show COVID-19-associated CD4+ lymphopenia and dysfunction is autocrine TNF-\u03b1\/TNFRI-dependent and therapies targeting TNF-\u03b1 may be beneficial in severe COVID-19.<\/span><\/p>\nShort CV<\/span><\/strong><\/p>\nI am a Research Associate Professor in the Division of Pulmonary, Allergy, and Critical Care Medicine at the University of Pittsburgh, and I have significant experience in transplant research in basic immunology with a focus on mechanisms of CMV-specific lung mucosal\/systemic immunity and viral control, as well on EBV, showing the important role for the Type-1 transcription factor T-bet and Eomes as well other immune markers. I also have experience in studying T cell mechanisms of allograft rejection\/tolerance in lung transplantation. I am the Co-I on a U01 award (AI125050) to perform a novel clinical trial in tandem lung and bone marrow transplantation in select individuals with primary immunodeficiencies and end-stage lung disease, and to perform mechanistic pathogen-specific and alloimmune tolerance studies in these study participants. I am also a Co-I on an R01 award HL133184-01, \u201cRole of F-box Proteins in Lung Transplantation.\u201d In my study I\u2019ve focused in viral mucosal immunology and I\u2019m the Co-I on U01HL121814, \u201cImmune Mechanisms of HIV-associated COPD. In addition, I\u2019m the Co-I in another lung transplant research effort focused on novel drug development for antibody-mediated lung transplant rejection (Amgen). At the University of Pittsburgh site I\u2019m Co-I for the R01, \u201cClinical Risk Factors in Primary Graft Dysfunction\u201d (HL087115). I am proficient in measuring lung mucosal and systemic viral-specific and allospecific multifunctional T cell immunity and phenotyping in lung transplant recipients. I have helped in the establishment of an extensive lung transplant tissue biorepository\/registry (BREATHE-LT) that collects and stores BAL fluid\/cells, blood for plasma\/PBMC, bronchial brushes, along with a clinical research database for phenotyping lung transplant recipients and assessing outcomes for translational studies. I am also working as a Co-PI on a current CFF award, \u201cMolecular and Clinical Endotypes in Chronic Lung Allograft Dysfunction\u201d in which RNA sequence analysis of the airway transcriptome and proteomics in ongoing studies in the mechanisms of CLAD. Finally, we have recently reported on the impact of short telomeres in pulmonary fibrosis lung transplant recipients who demonstrate increased CMV complications and impaired viral immunity. Pertinent to this proposal, recently I\u2019m Co-I working with Dr. McDyer and Dr. Callaway in performing the ELISA assay to determine SARS CoV-2 Spike protein titers and SARS CoV-2 neutralization assay titers to assess convalescent plasma units and the impact of convalescent plasma therapy in participants in this SIREN Network clinical trial.<\/span><\/p>\nPositions and Employment<\/strong><\/span>
\n2020-pres Research Associate Professor, Pulmonary, Allergy and Critical Care Division, Department Medicine, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2014-2020. Research Assistant Professor, Pulmonary, Allergy and Critical Care Division, Department Medicine, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2012-2014 Research Associate\/Manager Flow Cytometry Core, Pulmonary, Allergy and Critical Care Division, Department Medicine, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2010-2011 Senior Scientist\/Technical Application Specialist, Cellular Technology Limited, Shaker Heights, OH<\/span>
\n2006-2010 Faculty Research Instructor, Dept of Surgery Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2001-2006 Postdoctoral Research Associate, Dept of Surgery Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2000-2001 Research Scientist, Dept. of Cellular Immunology and Signal Transduction, Center of Immunology, Institute of Virology Bucharest, Romania<\/span>
\n1997- 2000 Junior Research Scientist, Department Clinical Immunology, Center for Immunology, Bucharest, Romania<\/span>
\n1997 3 months-Visiting Research PhD, Doctoral Scientist, Cancer Institute, University of Pittsburgh, Pittsburgh, PA.<\/span>
\n1994-1997 Research Associate – Dept. of Cellular Immunology and Signal Transduction, Center of Immunology, Institute of Virology, Bucharest, Romania<\/span>
\n1991-1994 Resident Scientist- Department Clinical Immunology, Center for Immunology, Bucharest, Romania<\/span>
\n1986-1991 Research<\/span>
\nMemberships<\/strong><\/span>
\nMember – American Society of Transplantation (AST) 2006-present<\/span>
\nMember – American Immunology Association (AIA) 2007-present<\/span>
\nMember – European Association for Cancer Research (EACR) 1998-present<\/span>
\nMember – Federation of European Biochemical Societies (FEBS) 1990-present<\/span>
\nMember – Romanian Society of Immunology (SNI) 1986-present<\/span>
\nMember \u2013 International Society of Heart and Lung Transplantation 2019-present<\/span><\/p>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.9.6″ background_color=”rgba(117,194,33,0.23)” background_image=”https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2021\/05\/PDI2021_header_1920x550px.jpg” background_blend=”multiply” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”97px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#75c221″ bottom_divider_flip=”vertical” bottom_divider_height_tablet=”” bottom_divider_height_phone=”59px” bottom_divider_height_last_edited=”on|desktop” global_module=”9177″ saved_tabs=”all”][et_pb_row column_structure=”3_5,2_5″ _builder_version=”3.25″ custom_padding=”61px|0px|69px|0px|false|false”][et_pb_column type=”3_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” text_line_height=”1.2em” header_font=”||||||||” background_layout=”dark” text_font_size_tablet=”” text_font_size_phone=”” text_font_size_last_edited=”on|desktop”]<\/p>\n
Contacteaz\u0103 operatorul PDI 2021<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@pdi.ro\/2021\/2019″ submit_button_text=”Trimite mesajul” _builder_version=”4.9.4″ title_font=”||||||||” custom_button=”on” button_text_color=”#F0622A”][et_pb_contact_field field_id=”Name” field_title=”Nume” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”Adresa de e-mail” field_type=”email” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Mesajul dvs.” field_type=”text” fullwidth_field=”on” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark”]<\/p>\n
Operatorul PDI 2021<\/p>\n
[\/et_pb_text][et_pb_text _builder_version=”4.0.11″ text_font=”||||||||”]<\/p>\n
![\"\"](\"https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2020\/02\/eventer_logo_150px.png\")
<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAdresa: Str. A. Panu nr. 13, Iasi<\/span>
Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off”]<\/p>\n
Parteneri Media<\/p>\n
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Prof. Univ. Dr. Iulia PopescuAllergy and Critical Care Division, Department Medicine, University of Pittsburgh, Pittsburgh, PACD4+ T cell lymphopenia and dysfunction in severe COVID-19 disease is autocrine TNF-\u03b1\/TNFRI-dependent Autori: Iulia Popescu, John F. McDyer One Sentence Summary: Autocrine TNF-\u03b1\/TNFRI regulates CD4+ T cell lymphopenia and dysfunction in severe COVID-19 disease. Lymphopenia is common in severe […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-8094","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8094","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/comments?post=8094"}],"version-history":[{"count":15,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8094\/revisions"}],"predecessor-version":[{"id":10149,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8094\/revisions\/10149"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/media?parent=8094"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Prof. Univ. Dr. Iulia Popescu<\/span><\/strong>
Allergy and Critical Care Division, Department Medicine, University of Pittsburgh, Pittsburgh, PA<\/span><\/strong><\/h4>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.6″]<\/p>\n
CD4+ T cell lymphopenia and dysfunction in severe COVID-19 disease is autocrine TNF-\u03b1\/TNFRI-dependent <\/span><\/strong>
\nAutori: Iulia Popescu, John F. McDyer<\/span><\/strong><\/p>\nOne Sentence Summary: Autocrine TNF-\u03b1\/TNFRI regulates CD4+ T cell lymphopenia and dysfunction in severe COVID-19 disease.<\/span><\/p>\nLymphopenia is common in severe COVID-19 disease, yet the mechanisms are poorly understood. In 148 patients with severe COVID-19, we found lymphopenia was associated with worse survival. CD4+ lymphopenia predominated, with lower CD4+\/CD8+ ratios in severe COVID-19 compared to recovered, mild disease (p<0.0001). In severe disease, immunodominant CD4+ T cell responses to Spike-1(S1) produced increased in vitro TNF-\u03b1, but impaired proliferation and increased susceptibility to activation-induced cell death (AICD). CD4+TNF-\u03b1+ T cell responses inversely correlated with absolute CD4+ counts from severe COVID-19 patients (n=76; R=-0.744, P<0.0001). TNF-\u03b1 blockade including infliximab or anti-TNFRI antibodies strikingly rescued S1-specific CD4+ proliferation and abrogated S1-AICD in severe COVID-19 patients (P<0.001). Single-cell RNAseq demonstrated downregulation of Type-1 cytokines and NFkB signaling in S1-stimulated CD4+ cells with infliximab treatment. Lung CD4+ T cells in severe COVID-19 were reduced and produced higher TNF-\u03b1 versus PBMC. Together, our findings show COVID-19-associated CD4+ lymphopenia and dysfunction is autocrine TNF-\u03b1\/TNFRI-dependent and therapies targeting TNF-\u03b1 may be beneficial in severe COVID-19.<\/span><\/p>\nShort CV<\/span><\/strong><\/p>\nI am a Research Associate Professor in the Division of Pulmonary, Allergy, and Critical Care Medicine at the University of Pittsburgh, and I have significant experience in transplant research in basic immunology with a focus on mechanisms of CMV-specific lung mucosal\/systemic immunity and viral control, as well on EBV, showing the important role for the Type-1 transcription factor T-bet and Eomes as well other immune markers. I also have experience in studying T cell mechanisms of allograft rejection\/tolerance in lung transplantation. I am the Co-I on a U01 award (AI125050) to perform a novel clinical trial in tandem lung and bone marrow transplantation in select individuals with primary immunodeficiencies and end-stage lung disease, and to perform mechanistic pathogen-specific and alloimmune tolerance studies in these study participants. I am also a Co-I on an R01 award HL133184-01, \u201cRole of F-box Proteins in Lung Transplantation.\u201d In my study I\u2019ve focused in viral mucosal immunology and I\u2019m the Co-I on U01HL121814, \u201cImmune Mechanisms of HIV-associated COPD. In addition, I\u2019m the Co-I in another lung transplant research effort focused on novel drug development for antibody-mediated lung transplant rejection (Amgen). At the University of Pittsburgh site I\u2019m Co-I for the R01, \u201cClinical Risk Factors in Primary Graft Dysfunction\u201d (HL087115). I am proficient in measuring lung mucosal and systemic viral-specific and allospecific multifunctional T cell immunity and phenotyping in lung transplant recipients. I have helped in the establishment of an extensive lung transplant tissue biorepository\/registry (BREATHE-LT) that collects and stores BAL fluid\/cells, blood for plasma\/PBMC, bronchial brushes, along with a clinical research database for phenotyping lung transplant recipients and assessing outcomes for translational studies. I am also working as a Co-PI on a current CFF award, \u201cMolecular and Clinical Endotypes in Chronic Lung Allograft Dysfunction\u201d in which RNA sequence analysis of the airway transcriptome and proteomics in ongoing studies in the mechanisms of CLAD. Finally, we have recently reported on the impact of short telomeres in pulmonary fibrosis lung transplant recipients who demonstrate increased CMV complications and impaired viral immunity. Pertinent to this proposal, recently I\u2019m Co-I working with Dr. McDyer and Dr. Callaway in performing the ELISA assay to determine SARS CoV-2 Spike protein titers and SARS CoV-2 neutralization assay titers to assess convalescent plasma units and the impact of convalescent plasma therapy in participants in this SIREN Network clinical trial.<\/span><\/p>\nPositions and Employment<\/strong><\/span>
\n2020-pres Research Associate Professor, Pulmonary, Allergy and Critical Care Division, Department Medicine, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2014-2020. Research Assistant Professor, Pulmonary, Allergy and Critical Care Division, Department Medicine, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2012-2014 Research Associate\/Manager Flow Cytometry Core, Pulmonary, Allergy and Critical Care Division, Department Medicine, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2010-2011 Senior Scientist\/Technical Application Specialist, Cellular Technology Limited, Shaker Heights, OH<\/span>
\n2006-2010 Faculty Research Instructor, Dept of Surgery Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2001-2006 Postdoctoral Research Associate, Dept of Surgery Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2000-2001 Research Scientist, Dept. of Cellular Immunology and Signal Transduction, Center of Immunology, Institute of Virology Bucharest, Romania<\/span>
\n1997- 2000 Junior Research Scientist, Department Clinical Immunology, Center for Immunology, Bucharest, Romania<\/span>
\n1997 3 months-Visiting Research PhD, Doctoral Scientist, Cancer Institute, University of Pittsburgh, Pittsburgh, PA.<\/span>
\n1994-1997 Research Associate – Dept. of Cellular Immunology and Signal Transduction, Center of Immunology, Institute of Virology, Bucharest, Romania<\/span>
\n1991-1994 Resident Scientist- Department Clinical Immunology, Center for Immunology, Bucharest, Romania<\/span>
\n1986-1991 Research<\/span>
\nMemberships<\/strong><\/span>
\nMember – American Society of Transplantation (AST) 2006-present<\/span>
\nMember – American Immunology Association (AIA) 2007-present<\/span>
\nMember – European Association for Cancer Research (EACR) 1998-present<\/span>
\nMember – Federation of European Biochemical Societies (FEBS) 1990-present<\/span>
\nMember – Romanian Society of Immunology (SNI) 1986-present<\/span>
\nMember \u2013 International Society of Heart and Lung Transplantation 2019-present<\/span><\/p>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.9.6″ background_color=”rgba(117,194,33,0.23)” background_image=”https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2021\/05\/PDI2021_header_1920x550px.jpg” background_blend=”multiply” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”97px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#75c221″ bottom_divider_flip=”vertical” bottom_divider_height_tablet=”” bottom_divider_height_phone=”59px” bottom_divider_height_last_edited=”on|desktop” global_module=”9177″ saved_tabs=”all”][et_pb_row column_structure=”3_5,2_5″ _builder_version=”3.25″ custom_padding=”61px|0px|69px|0px|false|false”][et_pb_column type=”3_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” text_line_height=”1.2em” header_font=”||||||||” background_layout=”dark” text_font_size_tablet=”” text_font_size_phone=”” text_font_size_last_edited=”on|desktop”]<\/p>\n
Contacteaz\u0103 operatorul PDI 2021<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@pdi.ro\/2021\/2019″ submit_button_text=”Trimite mesajul” _builder_version=”4.9.4″ title_font=”||||||||” custom_button=”on” button_text_color=”#F0622A”][et_pb_contact_field field_id=”Name” field_title=”Nume” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”Adresa de e-mail” field_type=”email” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Mesajul dvs.” field_type=”text” fullwidth_field=”on” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark”]<\/p>\n
Operatorul PDI 2021<\/p>\n
[\/et_pb_text][et_pb_text _builder_version=”4.0.11″ text_font=”||||||||”]<\/p>\n
<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAdresa: Str. A. Panu nr. 13, Iasi<\/span>
Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
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Prof. Univ. Dr. Iulia PopescuAllergy and Critical Care Division, Department Medicine, University of Pittsburgh, Pittsburgh, PACD4+ T cell lymphopenia and dysfunction in severe COVID-19 disease is autocrine TNF-\u03b1\/TNFRI-dependent Autori: Iulia Popescu, John F. McDyer One Sentence Summary: Autocrine TNF-\u03b1\/TNFRI regulates CD4+ T cell lymphopenia and dysfunction in severe COVID-19 disease. Lymphopenia is common in severe […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-8094","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8094","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/comments?post=8094"}],"version-history":[{"count":15,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8094\/revisions"}],"predecessor-version":[{"id":10149,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8094\/revisions\/10149"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/media?parent=8094"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nAutori: Iulia Popescu, John F. McDyer<\/span><\/strong><\/p>\n
One Sentence Summary: Autocrine TNF-\u03b1\/TNFRI regulates CD4+ T cell lymphopenia and dysfunction in severe COVID-19 disease.<\/span><\/p>\n Lymphopenia is common in severe COVID-19 disease, yet the mechanisms are poorly understood. In 148 patients with severe COVID-19, we found lymphopenia was associated with worse survival. CD4+ lymphopenia predominated, with lower CD4+\/CD8+ ratios in severe COVID-19 compared to recovered, mild disease (p<0.0001). In severe disease, immunodominant CD4+ T cell responses to Spike-1(S1) produced increased in vitro TNF-\u03b1, but impaired proliferation and increased susceptibility to activation-induced cell death (AICD). CD4+TNF-\u03b1+ T cell responses inversely correlated with absolute CD4+ counts from severe COVID-19 patients (n=76; R=-0.744, P<0.0001). TNF-\u03b1 blockade including infliximab or anti-TNFRI antibodies strikingly rescued S1-specific CD4+ proliferation and abrogated S1-AICD in severe COVID-19 patients (P<0.001). Single-cell RNAseq demonstrated downregulation of Type-1 cytokines and NFkB signaling in S1-stimulated CD4+ cells with infliximab treatment. Lung CD4+ T cells in severe COVID-19 were reduced and produced higher TNF-\u03b1 versus PBMC. Together, our findings show COVID-19-associated CD4+ lymphopenia and dysfunction is autocrine TNF-\u03b1\/TNFRI-dependent and therapies targeting TNF-\u03b1 may be beneficial in severe COVID-19.<\/span><\/p>\n Short CV<\/span><\/strong><\/p>\n I am a Research Associate Professor in the Division of Pulmonary, Allergy, and Critical Care Medicine at the University of Pittsburgh, and I have significant experience in transplant research in basic immunology with a focus on mechanisms of CMV-specific lung mucosal\/systemic immunity and viral control, as well on EBV, showing the important role for the Type-1 transcription factor T-bet and Eomes as well other immune markers. I also have experience in studying T cell mechanisms of allograft rejection\/tolerance in lung transplantation. I am the Co-I on a U01 award (AI125050) to perform a novel clinical trial in tandem lung and bone marrow transplantation in select individuals with primary immunodeficiencies and end-stage lung disease, and to perform mechanistic pathogen-specific and alloimmune tolerance studies in these study participants. I am also a Co-I on an R01 award HL133184-01, \u201cRole of F-box Proteins in Lung Transplantation.\u201d In my study I\u2019ve focused in viral mucosal immunology and I\u2019m the Co-I on U01HL121814, \u201cImmune Mechanisms of HIV-associated COPD. In addition, I\u2019m the Co-I in another lung transplant research effort focused on novel drug development for antibody-mediated lung transplant rejection (Amgen). At the University of Pittsburgh site I\u2019m Co-I for the R01, \u201cClinical Risk Factors in Primary Graft Dysfunction\u201d (HL087115). I am proficient in measuring lung mucosal and systemic viral-specific and allospecific multifunctional T cell immunity and phenotyping in lung transplant recipients. I have helped in the establishment of an extensive lung transplant tissue biorepository\/registry (BREATHE-LT) that collects and stores BAL fluid\/cells, blood for plasma\/PBMC, bronchial brushes, along with a clinical research database for phenotyping lung transplant recipients and assessing outcomes for translational studies. I am also working as a Co-PI on a current CFF award, \u201cMolecular and Clinical Endotypes in Chronic Lung Allograft Dysfunction\u201d in which RNA sequence analysis of the airway transcriptome and proteomics in ongoing studies in the mechanisms of CLAD. Finally, we have recently reported on the impact of short telomeres in pulmonary fibrosis lung transplant recipients who demonstrate increased CMV complications and impaired viral immunity. Pertinent to this proposal, recently I\u2019m Co-I working with Dr. McDyer and Dr. Callaway in performing the ELISA assay to determine SARS CoV-2 Spike protein titers and SARS CoV-2 neutralization assay titers to assess convalescent plasma units and the impact of convalescent plasma therapy in participants in this SIREN Network clinical trial.<\/span><\/p>\n Positions and Employment<\/strong><\/span> [\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.9.6″ background_color=”rgba(117,194,33,0.23)” background_image=”https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2021\/05\/PDI2021_header_1920x550px.jpg” background_blend=”multiply” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”97px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#75c221″ 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McDyer One Sentence Summary: Autocrine TNF-\u03b1\/TNFRI regulates CD4+ T cell lymphopenia and dysfunction in severe COVID-19 disease. Lymphopenia is common in severe […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-8094","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8094","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/comments?post=8094"}],"version-history":[{"count":15,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8094\/revisions"}],"predecessor-version":[{"id":10149,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8094\/revisions\/10149"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/media?parent=8094"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\n2020-pres Research Associate Professor, Pulmonary, Allergy and Critical Care Division, Department Medicine, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2014-2020. Research Assistant Professor, Pulmonary, Allergy and Critical Care Division, Department Medicine, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2012-2014 Research Associate\/Manager Flow Cytometry Core, Pulmonary, Allergy and Critical Care Division, Department Medicine, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2010-2011 Senior Scientist\/Technical Application Specialist, Cellular Technology Limited, Shaker Heights, OH<\/span>
\n2006-2010 Faculty Research Instructor, Dept of Surgery Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2001-2006 Postdoctoral Research Associate, Dept of Surgery Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA<\/span>
\n2000-2001 Research Scientist, Dept. of Cellular Immunology and Signal Transduction, Center of Immunology, Institute of Virology Bucharest, Romania<\/span>
\n1997- 2000 Junior Research Scientist, Department Clinical Immunology, Center for Immunology, Bucharest, Romania<\/span>
\n1997 3 months-Visiting Research PhD, Doctoral Scientist, Cancer Institute, University of Pittsburgh, Pittsburgh, PA.<\/span>
\n1994-1997 Research Associate – Dept. of Cellular Immunology and Signal Transduction, Center of Immunology, Institute of Virology, Bucharest, Romania<\/span>
\n1991-1994 Resident Scientist- Department Clinical Immunology, Center for Immunology, Bucharest, Romania<\/span>
\n1986-1991 Research<\/span>
\nMemberships<\/strong><\/span>
\nMember – American Society of Transplantation (AST) 2006-present<\/span>
\nMember – American Immunology Association (AIA) 2007-present<\/span>
\nMember – European Association for Cancer Research (EACR) 1998-present<\/span>
\nMember – Federation of European Biochemical Societies (FEBS) 1990-present<\/span>
\nMember – Romanian Society of Immunology (SNI) 1986-present<\/span>
\nMember \u2013 International Society of Heart and Lung Transplantation 2019-present<\/span><\/p>\n<\/span><\/p>\n
Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n