<\/strong><\/span><\/h5>\n<\/strong><\/span><\/h5>\n<\/strong><\/span><\/h5>\n<\/strong><\/span><\/h5>\n\u015eef lucr. Dr. B\u0103descu Aida Corina<\/strong><\/span>
Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fi<\/strong><\/span><\/h5>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ locked=”off”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.7″ hover_enabled=”0″ sticky_enabled=”0″]<\/p>\n
Diagnosticul molecular \u00een infec\u021bia SARS-CoV-2, de la perfec\u021biunea tehnicii la arta interpret\u0103rii rezultatelor<\/span>
Autor: Aida Corina B\u0103descu<\/span><\/p>\nCuvinte cheie: virus SARS-CoV-2, diagnostic de laborator, metode moleculare<\/p>\n
Sopul acestei prezent\u0103ri este acela de a oferi participan\u021bilor o privire de ansamblu asupra posibilit\u0103\u021bilor actulale de diagnostic molecular \u00een infec\u021bia cu virusul SARS-CoV-2.
Infec\u021bia cu virusul SARS-CoV-2 a reprezentat o imens\u0103 provocare pentru medicina rom\u00e2neasc\u0103 \u0219i din punctul de vedere al posibilit\u0103\u021bilor de diagnostic etiologic. Tehnicile de diagnostic molecular erau utilizate de rutin\u0103 la noi \u00een \u021bar\u0103 doar pentru diagnosticul \u0219i monitorizarea unor infec\u021bii virale care beneficiau de terapie specific\u0103 (infec\u021bia HIV, infec\u021bia cu virusuri hepatitice) sau pentru diagnosticul tuberculozei. Extinderea rapid\u0103 a capacit\u0103\u021bilor de diagnostic molecular a reprezentat cea mai mare provocare pentru medicina de laborator, la \u00eenceputul pandemiei fiind necesar\u0103 at\u00e2t dezvoltarea mijloacelor tehnice c\u00e2t \u0219i preg\u0103tirea specific\u0103 a personalului pentru realizarea acestor teste ce presupun un nivel superior de preg\u0103tire al personalului din laborator.
Metodele moderne de diagnostic (hibridizare, tehnici de amplificare genic\u0103 \u2013 PCR, secven\u021biere), bazate pe detec\u021bia genelor specifice acestui patogen, pun la dispozi\u021bia laboratorului \u0219i implicit a clinicianului cel mai valoros instrument pentru detec\u021bia rapid\u0103, foarte sensibil\u0103 \u0219i foarte specific\u0103 a prezen\u021bei virusului dar permit \u0219i o apreciere cantitativ\u0103 sau semicantitativ\u0103 a nivelului s\u0103u \u00een secre\u021biile respiratorii, cu impact major \u00een rata de transmitere la contac\u021bi. Apari\u021bia inerent\u0103 a muta\u021biilor virusului, favorizat\u0103 de num\u0103rul foarte mare de persoane infectate la nivel global, a generat \u00een plus nevoia de urm\u0103rire a acestor muta\u021bii \u0219i a variantelor virale emergente, tot prin tehnici moleculare de PCR sau de secven\u021biere genomic\u0103.
![](\"https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2021\/07\/image_3.jpg\")
Prezentarea va include o trecere \u00een revist\u0103 a testelor de diagnostic molecular utilizate \u00een diagnosticul infec\u021biei cu virusul SARS-CoV-2, cu avantajele \u0219i cu dezavantajele lor dar \u0219i cu problemele de interpretare pe care le ridic\u0103.<\/p>\n
Molecular diagnosis in SARS-CoV-2 infection, from technical perfection to the art of test rezults interpretation<\/span>
Autor: Aida Corina B\u0103descu
<\/span><\/p>\nKeywords: SARS-CoV-2 virus, laboratory diagnosis, molecular methods<\/p>\n
The purpose of this presentation is to provide participants with an overview of the current possibilities of molecular diagnosis in SARS-CoV-2 infection.
The infection with the SARS-CoV-2 virus represented an huge challenge for the romanian medicine also from the point of view of the etiological diagnosis possibilities. Molecular diagnostic techniques were routinely used in our country only for the diagnosis and monitoring of viral infections that received specific therapy (HIV infection, viral hepatitis) or for the diagnosis of tuberculosis. The rapid expansion of molecular diagnostic capabilities was the biggest challenge for laboratory medicine, at the beginning of the pandemic being necessary both the development of technical means and specific training of staff to perform these tests that require a higher level of training of laboratory staff.
Modern diagnostic methods (hybridization, gene amplification \u2013 PCR, sequencing), based on the detection of genes specific to this pathogen, provide the laboratory and the clinician with the most valuable tool for rapid, very sensitive and very specific detection of the virus but also allow a quantitative or semi-quantitative assessment of its level in respiratory secretions, with a major impact on the rate of transmission to contacts. The inherent appearance of virus mutations, favored by the very large number of infected people globally, has also generated the need to track these mutations and emerging viral variants, also through molecular techniques of PCR or genomic sequencing.
The presentation will include a review of the molecular diagnostic tests used in the diagnosis of SARS-CoV-2 virus infection, with their advantages and disadvantages but also with the interpretation problems they raise.<\/p>\n
Scurt CV<\/span><\/p>\n\u015eef lucr\u0103ri Dr. Aida Corina B\u0103descu
Disciplina de Microbiologie
Facultatea de Medicin\u0103
U.M.F. \u201dGrigore T. Popa\u201d Ia\u015fi<\/p>\n
Medic primar Medicin\u0103 de Laborator
Doctor \u00een \u015ftiin\u0163e medicale \u2013 specialitatea Dermatovenerologie.
Medic Coordonator Laboratorul de Biologie Molecular\u0103, Spitalul de Boli Infec\u0163ioase “Sf. Parascheva\u201d Ia\u015fi<\/p>\n
Membru \u00een societ\u0103\u0163i \u015ftiin\u0163ifice:
\u2022 European Society for Clinical Microbiology and Infectious Disease
\u2022 Societatea Rom\u00e2n\u0103 de Microbiologie
\u2022 Societatea Rom\u00e2n\u0103 de Imunologie
\u2022 Societatea Rom\u00e2n\u0103 de Micologie<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.9.6″ background_color=”rgba(117,194,33,0.23)” background_image=”https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2021\/05\/PDI2021_header_1920x550px.jpg” background_blend=”multiply” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”97px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#75c221″ bottom_divider_flip=”vertical” bottom_divider_height_tablet=”” bottom_divider_height_phone=”59px” bottom_divider_height_last_edited=”on|desktop” saved_tabs=”all” global_module=”9177″][et_pb_row column_structure=”3_5,2_5″ _builder_version=”3.25″ custom_padding=”61px|0px|69px|0px|false|false”][et_pb_column type=”3_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” text_line_height=”1.2em” header_font=”||||||||” background_layout=”dark” text_font_size_tablet=”” text_font_size_phone=”” text_font_size_last_edited=”on|desktop”]<\/p>\n
Contacteaz\u0103 operatorul PDI 2021<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@pdi.ro\/2021\/2019″ submit_button_text=”Trimite mesajul” _builder_version=”4.9.4″ title_font=”||||||||” custom_button=”on” button_text_color=”#F0622A”][et_pb_contact_field field_id=”Name” field_title=”Nume” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”Adresa de e-mail” field_type=”email” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Mesajul dvs.” field_type=”text” fullwidth_field=”on” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark”]<\/p>\n
Operatorul PDI 2021<\/p>\n
[\/et_pb_text][et_pb_text _builder_version=”4.0.11″ text_font=”||||||||”]<\/p>\n
![\"\"](\"https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2020\/02\/eventer_logo_150px.png\")
<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAdresa: Str. A. Panu nr. 13, Iasi<\/span>
Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off”]<\/p>\n
Parteneri Media<\/p>\n
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\u015eef lucr. Dr. B\u0103descu Aida Corina Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fiDiagnosticul molecular \u00een infec\u021bia SARS-CoV-2, de la perfec\u021biunea tehnicii la arta interpret\u0103rii rezultatelor Autor: Aida Corina B\u0103descu Cuvinte cheie: virus SARS-CoV-2, diagnostic de laborator, metode moleculare Sopul acestei prezent\u0103ri este acela de a oferi participan\u021bilor o privire de ansamblu asupra posibilit\u0103\u021bilor […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-8649","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8649","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/comments?post=8649"}],"version-history":[{"count":5,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8649\/revisions"}],"predecessor-version":[{"id":10553,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8649\/revisions\/10553"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/media?parent=8649"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/strong><\/span><\/h5>\n<\/strong><\/span><\/h5>\n\u015eef lucr. Dr. B\u0103descu Aida Corina<\/strong><\/span>
Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fi<\/strong><\/span><\/h5>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ locked=”off”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.7″ hover_enabled=”0″ sticky_enabled=”0″]<\/p>\n
Diagnosticul molecular \u00een infec\u021bia SARS-CoV-2, de la perfec\u021biunea tehnicii la arta interpret\u0103rii rezultatelor<\/span>
Autor: Aida Corina B\u0103descu<\/span><\/p>\nCuvinte cheie: virus SARS-CoV-2, diagnostic de laborator, metode moleculare<\/p>\n
Sopul acestei prezent\u0103ri este acela de a oferi participan\u021bilor o privire de ansamblu asupra posibilit\u0103\u021bilor actulale de diagnostic molecular \u00een infec\u021bia cu virusul SARS-CoV-2.
Infec\u021bia cu virusul SARS-CoV-2 a reprezentat o imens\u0103 provocare pentru medicina rom\u00e2neasc\u0103 \u0219i din punctul de vedere al posibilit\u0103\u021bilor de diagnostic etiologic. Tehnicile de diagnostic molecular erau utilizate de rutin\u0103 la noi \u00een \u021bar\u0103 doar pentru diagnosticul \u0219i monitorizarea unor infec\u021bii virale care beneficiau de terapie specific\u0103 (infec\u021bia HIV, infec\u021bia cu virusuri hepatitice) sau pentru diagnosticul tuberculozei. Extinderea rapid\u0103 a capacit\u0103\u021bilor de diagnostic molecular a reprezentat cea mai mare provocare pentru medicina de laborator, la \u00eenceputul pandemiei fiind necesar\u0103 at\u00e2t dezvoltarea mijloacelor tehnice c\u00e2t \u0219i preg\u0103tirea specific\u0103 a personalului pentru realizarea acestor teste ce presupun un nivel superior de preg\u0103tire al personalului din laborator.
Metodele moderne de diagnostic (hibridizare, tehnici de amplificare genic\u0103 \u2013 PCR, secven\u021biere), bazate pe detec\u021bia genelor specifice acestui patogen, pun la dispozi\u021bia laboratorului \u0219i implicit a clinicianului cel mai valoros instrument pentru detec\u021bia rapid\u0103, foarte sensibil\u0103 \u0219i foarte specific\u0103 a prezen\u021bei virusului dar permit \u0219i o apreciere cantitativ\u0103 sau semicantitativ\u0103 a nivelului s\u0103u \u00een secre\u021biile respiratorii, cu impact major \u00een rata de transmitere la contac\u021bi. Apari\u021bia inerent\u0103 a muta\u021biilor virusului, favorizat\u0103 de num\u0103rul foarte mare de persoane infectate la nivel global, a generat \u00een plus nevoia de urm\u0103rire a acestor muta\u021bii \u0219i a variantelor virale emergente, tot prin tehnici moleculare de PCR sau de secven\u021biere genomic\u0103.
Prezentarea va include o trecere \u00een revist\u0103 a testelor de diagnostic molecular utilizate \u00een diagnosticul infec\u021biei cu virusul SARS-CoV-2, cu avantajele \u0219i cu dezavantajele lor dar \u0219i cu problemele de interpretare pe care le ridic\u0103.<\/p>\n
Molecular diagnosis in SARS-CoV-2 infection, from technical perfection to the art of test rezults interpretation<\/span>
Autor: Aida Corina B\u0103descu
<\/span><\/p>\nKeywords: SARS-CoV-2 virus, laboratory diagnosis, molecular methods<\/p>\n
The purpose of this presentation is to provide participants with an overview of the current possibilities of molecular diagnosis in SARS-CoV-2 infection.
The infection with the SARS-CoV-2 virus represented an huge challenge for the romanian medicine also from the point of view of the etiological diagnosis possibilities. Molecular diagnostic techniques were routinely used in our country only for the diagnosis and monitoring of viral infections that received specific therapy (HIV infection, viral hepatitis) or for the diagnosis of tuberculosis. The rapid expansion of molecular diagnostic capabilities was the biggest challenge for laboratory medicine, at the beginning of the pandemic being necessary both the development of technical means and specific training of staff to perform these tests that require a higher level of training of laboratory staff.
Modern diagnostic methods (hybridization, gene amplification \u2013 PCR, sequencing), based on the detection of genes specific to this pathogen, provide the laboratory and the clinician with the most valuable tool for rapid, very sensitive and very specific detection of the virus but also allow a quantitative or semi-quantitative assessment of its level in respiratory secretions, with a major impact on the rate of transmission to contacts. The inherent appearance of virus mutations, favored by the very large number of infected people globally, has also generated the need to track these mutations and emerging viral variants, also through molecular techniques of PCR or genomic sequencing.
The presentation will include a review of the molecular diagnostic tests used in the diagnosis of SARS-CoV-2 virus infection, with their advantages and disadvantages but also with the interpretation problems they raise.<\/p>\n
Scurt CV<\/span><\/p>\n\u015eef lucr\u0103ri Dr. Aida Corina B\u0103descu
Disciplina de Microbiologie
Facultatea de Medicin\u0103
U.M.F. \u201dGrigore T. Popa\u201d Ia\u015fi<\/p>\n
Medic primar Medicin\u0103 de Laborator
Doctor \u00een \u015ftiin\u0163e medicale \u2013 specialitatea Dermatovenerologie.
Medic Coordonator Laboratorul de Biologie Molecular\u0103, Spitalul de Boli Infec\u0163ioase “Sf. Parascheva\u201d Ia\u015fi<\/p>\n
Membru \u00een societ\u0103\u0163i \u015ftiin\u0163ifice:
\u2022 European Society for Clinical Microbiology and Infectious Disease
\u2022 Societatea Rom\u00e2n\u0103 de Microbiologie
\u2022 Societatea Rom\u00e2n\u0103 de Imunologie
\u2022 Societatea Rom\u00e2n\u0103 de Micologie<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.9.6″ background_color=”rgba(117,194,33,0.23)” background_image=”https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2021\/05\/PDI2021_header_1920x550px.jpg” background_blend=”multiply” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”97px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#75c221″ bottom_divider_flip=”vertical” bottom_divider_height_tablet=”” bottom_divider_height_phone=”59px” bottom_divider_height_last_edited=”on|desktop” saved_tabs=”all” global_module=”9177″][et_pb_row column_structure=”3_5,2_5″ _builder_version=”3.25″ custom_padding=”61px|0px|69px|0px|false|false”][et_pb_column type=”3_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” text_line_height=”1.2em” header_font=”||||||||” background_layout=”dark” text_font_size_tablet=”” text_font_size_phone=”” text_font_size_last_edited=”on|desktop”]<\/p>\n
Contacteaz\u0103 operatorul PDI 2021<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@pdi.ro\/2021\/2019″ submit_button_text=”Trimite mesajul” _builder_version=”4.9.4″ title_font=”||||||||” custom_button=”on” button_text_color=”#F0622A”][et_pb_contact_field field_id=”Name” field_title=”Nume” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”Adresa de e-mail” field_type=”email” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Mesajul dvs.” field_type=”text” fullwidth_field=”on” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark”]<\/p>\n
Operatorul PDI 2021<\/p>\n
[\/et_pb_text][et_pb_text _builder_version=”4.0.11″ text_font=”||||||||”]<\/p>\n
<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAdresa: Str. A. Panu nr. 13, Iasi<\/span>
Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off”]<\/p>\n
Parteneri Media<\/p>\n
[\/et_pb_text][et_pb_gallery gallery_ids=”7203,7204,9252,9464,10156,10157″ fullwidth=”on” _builder_version=”4.9.7″ transform_scale=”86%|86%” max_width=”63%” auto=”on” auto_speed=”3500″ hover_transition_duration=”100ms” hover_transition_delay=”50ms” hover_transition_speed_curve=”ease-in-out” locked=”off”][\/et_pb_gallery][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"
\u015eef lucr. Dr. B\u0103descu Aida Corina Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fiDiagnosticul molecular \u00een infec\u021bia SARS-CoV-2, de la perfec\u021biunea tehnicii la arta interpret\u0103rii rezultatelor Autor: Aida Corina B\u0103descu Cuvinte cheie: virus SARS-CoV-2, diagnostic de laborator, metode moleculare Sopul acestei prezent\u0103ri este acela de a oferi participan\u021bilor o privire de ansamblu asupra posibilit\u0103\u021bilor […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-8649","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8649","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/comments?post=8649"}],"version-history":[{"count":5,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8649\/revisions"}],"predecessor-version":[{"id":10553,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8649\/revisions\/10553"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/media?parent=8649"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\u015eef lucr. Dr. B\u0103descu Aida Corina<\/strong><\/span>
Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fi<\/strong><\/span><\/h5>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″ locked=”off”][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.7″ hover_enabled=”0″ sticky_enabled=”0″]<\/p>\n
Diagnosticul molecular \u00een infec\u021bia SARS-CoV-2, de la perfec\u021biunea tehnicii la arta interpret\u0103rii rezultatelor<\/span>
Autor: Aida Corina B\u0103descu<\/span><\/p>\nCuvinte cheie: virus SARS-CoV-2, diagnostic de laborator, metode moleculare<\/p>\n
Sopul acestei prezent\u0103ri este acela de a oferi participan\u021bilor o privire de ansamblu asupra posibilit\u0103\u021bilor actulale de diagnostic molecular \u00een infec\u021bia cu virusul SARS-CoV-2.
Infec\u021bia cu virusul SARS-CoV-2 a reprezentat o imens\u0103 provocare pentru medicina rom\u00e2neasc\u0103 \u0219i din punctul de vedere al posibilit\u0103\u021bilor de diagnostic etiologic. Tehnicile de diagnostic molecular erau utilizate de rutin\u0103 la noi \u00een \u021bar\u0103 doar pentru diagnosticul \u0219i monitorizarea unor infec\u021bii virale care beneficiau de terapie specific\u0103 (infec\u021bia HIV, infec\u021bia cu virusuri hepatitice) sau pentru diagnosticul tuberculozei. Extinderea rapid\u0103 a capacit\u0103\u021bilor de diagnostic molecular a reprezentat cea mai mare provocare pentru medicina de laborator, la \u00eenceputul pandemiei fiind necesar\u0103 at\u00e2t dezvoltarea mijloacelor tehnice c\u00e2t \u0219i preg\u0103tirea specific\u0103 a personalului pentru realizarea acestor teste ce presupun un nivel superior de preg\u0103tire al personalului din laborator.
Metodele moderne de diagnostic (hibridizare, tehnici de amplificare genic\u0103 \u2013 PCR, secven\u021biere), bazate pe detec\u021bia genelor specifice acestui patogen, pun la dispozi\u021bia laboratorului \u0219i implicit a clinicianului cel mai valoros instrument pentru detec\u021bia rapid\u0103, foarte sensibil\u0103 \u0219i foarte specific\u0103 a prezen\u021bei virusului dar permit \u0219i o apreciere cantitativ\u0103 sau semicantitativ\u0103 a nivelului s\u0103u \u00een secre\u021biile respiratorii, cu impact major \u00een rata de transmitere la contac\u021bi. Apari\u021bia inerent\u0103 a muta\u021biilor virusului, favorizat\u0103 de num\u0103rul foarte mare de persoane infectate la nivel global, a generat \u00een plus nevoia de urm\u0103rire a acestor muta\u021bii \u0219i a variantelor virale emergente, tot prin tehnici moleculare de PCR sau de secven\u021biere genomic\u0103.
Prezentarea va include o trecere \u00een revist\u0103 a testelor de diagnostic molecular utilizate \u00een diagnosticul infec\u021biei cu virusul SARS-CoV-2, cu avantajele \u0219i cu dezavantajele lor dar \u0219i cu problemele de interpretare pe care le ridic\u0103.<\/p>\n
Molecular diagnosis in SARS-CoV-2 infection, from technical perfection to the art of test rezults interpretation<\/span>
Autor: Aida Corina B\u0103descu
<\/span><\/p>\nKeywords: SARS-CoV-2 virus, laboratory diagnosis, molecular methods<\/p>\n
The purpose of this presentation is to provide participants with an overview of the current possibilities of molecular diagnosis in SARS-CoV-2 infection.
The infection with the SARS-CoV-2 virus represented an huge challenge for the romanian medicine also from the point of view of the etiological diagnosis possibilities. Molecular diagnostic techniques were routinely used in our country only for the diagnosis and monitoring of viral infections that received specific therapy (HIV infection, viral hepatitis) or for the diagnosis of tuberculosis. The rapid expansion of molecular diagnostic capabilities was the biggest challenge for laboratory medicine, at the beginning of the pandemic being necessary both the development of technical means and specific training of staff to perform these tests that require a higher level of training of laboratory staff.
Modern diagnostic methods (hybridization, gene amplification \u2013 PCR, sequencing), based on the detection of genes specific to this pathogen, provide the laboratory and the clinician with the most valuable tool for rapid, very sensitive and very specific detection of the virus but also allow a quantitative or semi-quantitative assessment of its level in respiratory secretions, with a major impact on the rate of transmission to contacts. The inherent appearance of virus mutations, favored by the very large number of infected people globally, has also generated the need to track these mutations and emerging viral variants, also through molecular techniques of PCR or genomic sequencing.
The presentation will include a review of the molecular diagnostic tests used in the diagnosis of SARS-CoV-2 virus infection, with their advantages and disadvantages but also with the interpretation problems they raise.<\/p>\n
Scurt CV<\/span><\/p>\n\u015eef lucr\u0103ri Dr. Aida Corina B\u0103descu
Disciplina de Microbiologie
Facultatea de Medicin\u0103
U.M.F. \u201dGrigore T. Popa\u201d Ia\u015fi<\/p>\n
Medic primar Medicin\u0103 de Laborator
Doctor \u00een \u015ftiin\u0163e medicale \u2013 specialitatea Dermatovenerologie.
Medic Coordonator Laboratorul de Biologie Molecular\u0103, Spitalul de Boli Infec\u0163ioase “Sf. Parascheva\u201d Ia\u015fi<\/p>\n
Membru \u00een societ\u0103\u0163i \u015ftiin\u0163ifice:
\u2022 European Society for Clinical Microbiology and Infectious Disease
\u2022 Societatea Rom\u00e2n\u0103 de Microbiologie
\u2022 Societatea Rom\u00e2n\u0103 de Imunologie
\u2022 Societatea Rom\u00e2n\u0103 de Micologie<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.9.6″ background_color=”rgba(117,194,33,0.23)” background_image=”https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2021\/05\/PDI2021_header_1920x550px.jpg” background_blend=”multiply” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”97px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#75c221″ bottom_divider_flip=”vertical” bottom_divider_height_tablet=”” bottom_divider_height_phone=”59px” bottom_divider_height_last_edited=”on|desktop” saved_tabs=”all” global_module=”9177″][et_pb_row column_structure=”3_5,2_5″ _builder_version=”3.25″ custom_padding=”61px|0px|69px|0px|false|false”][et_pb_column type=”3_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” text_line_height=”1.2em” header_font=”||||||||” background_layout=”dark” text_font_size_tablet=”” text_font_size_phone=”” text_font_size_last_edited=”on|desktop”]<\/p>\n
Contacteaz\u0103 operatorul PDI 2021<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@pdi.ro\/2021\/2019″ submit_button_text=”Trimite mesajul” _builder_version=”4.9.4″ title_font=”||||||||” custom_button=”on” button_text_color=”#F0622A”][et_pb_contact_field field_id=”Name” field_title=”Nume” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”Adresa de e-mail” field_type=”email” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Mesajul dvs.” field_type=”text” fullwidth_field=”on” _builder_version=”3.18.9″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.9.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark”]<\/p>\n
Operatorul PDI 2021<\/p>\n
[\/et_pb_text][et_pb_text _builder_version=”4.0.11″ text_font=”||||||||”]<\/p>\n
<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAdresa: Str. A. Panu nr. 13, Iasi<\/span>
Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off”]<\/p>\n
Parteneri Media<\/p>\n
[\/et_pb_text][et_pb_gallery gallery_ids=”7203,7204,9252,9464,10156,10157″ fullwidth=”on” _builder_version=”4.9.7″ transform_scale=”86%|86%” max_width=”63%” auto=”on” auto_speed=”3500″ hover_transition_duration=”100ms” hover_transition_delay=”50ms” hover_transition_speed_curve=”ease-in-out” locked=”off”][\/et_pb_gallery][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"
\u015eef lucr. Dr. B\u0103descu Aida Corina Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fiDiagnosticul molecular \u00een infec\u021bia SARS-CoV-2, de la perfec\u021biunea tehnicii la arta interpret\u0103rii rezultatelor Autor: Aida Corina B\u0103descu Cuvinte cheie: virus SARS-CoV-2, diagnostic de laborator, metode moleculare Sopul acestei prezent\u0103ri este acela de a oferi participan\u021bilor o privire de ansamblu asupra posibilit\u0103\u021bilor […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-8649","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8649","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/comments?post=8649"}],"version-history":[{"count":5,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8649\/revisions"}],"predecessor-version":[{"id":10553,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8649\/revisions\/10553"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/media?parent=8649"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Autor: Aida Corina B\u0103descu<\/span><\/p>\n
Cuvinte cheie: virus SARS-CoV-2, diagnostic de laborator, metode moleculare<\/p>\n
Sopul acestei prezent\u0103ri este acela de a oferi participan\u021bilor o privire de ansamblu asupra posibilit\u0103\u021bilor actulale de diagnostic molecular \u00een infec\u021bia cu virusul SARS-CoV-2.
Infec\u021bia cu virusul SARS-CoV-2 a reprezentat o imens\u0103 provocare pentru medicina rom\u00e2neasc\u0103 \u0219i din punctul de vedere al posibilit\u0103\u021bilor de diagnostic etiologic. Tehnicile de diagnostic molecular erau utilizate de rutin\u0103 la noi \u00een \u021bar\u0103 doar pentru diagnosticul \u0219i monitorizarea unor infec\u021bii virale care beneficiau de terapie specific\u0103 (infec\u021bia HIV, infec\u021bia cu virusuri hepatitice) sau pentru diagnosticul tuberculozei. Extinderea rapid\u0103 a capacit\u0103\u021bilor de diagnostic molecular a reprezentat cea mai mare provocare pentru medicina de laborator, la \u00eenceputul pandemiei fiind necesar\u0103 at\u00e2t dezvoltarea mijloacelor tehnice c\u00e2t \u0219i preg\u0103tirea specific\u0103 a personalului pentru realizarea acestor teste ce presupun un nivel superior de preg\u0103tire al personalului din laborator.
Metodele moderne de diagnostic (hibridizare, tehnici de amplificare genic\u0103 \u2013 PCR, secven\u021biere), bazate pe detec\u021bia genelor specifice acestui patogen, pun la dispozi\u021bia laboratorului \u0219i implicit a clinicianului cel mai valoros instrument pentru detec\u021bia rapid\u0103, foarte sensibil\u0103 \u0219i foarte specific\u0103 a prezen\u021bei virusului dar permit \u0219i o apreciere cantitativ\u0103 sau semicantitativ\u0103 a nivelului s\u0103u \u00een secre\u021biile respiratorii, cu impact major \u00een rata de transmitere la contac\u021bi. Apari\u021bia inerent\u0103 a muta\u021biilor virusului, favorizat\u0103 de num\u0103rul foarte mare de persoane infectate la nivel global, a generat \u00een plus nevoia de urm\u0103rire a acestor muta\u021bii \u0219i a variantelor virale emergente, tot prin tehnici moleculare de PCR sau de secven\u021biere genomic\u0103.
Prezentarea va include o trecere \u00een revist\u0103 a testelor de diagnostic molecular utilizate \u00een diagnosticul infec\u021biei cu virusul SARS-CoV-2, cu avantajele \u0219i cu dezavantajele lor dar \u0219i cu problemele de interpretare pe care le ridic\u0103.<\/p>\n
Molecular diagnosis in SARS-CoV-2 infection, from technical perfection to the art of test rezults interpretation<\/span> Keywords: SARS-CoV-2 virus, laboratory diagnosis, molecular methods<\/p>\n The purpose of this presentation is to provide participants with an overview of the current possibilities of molecular diagnosis in SARS-CoV-2 infection. Scurt CV<\/span><\/p>\n \u015eef lucr\u0103ri Dr. Aida Corina B\u0103descu Medic primar Medicin\u0103 de Laborator Membru \u00een societ\u0103\u0163i \u015ftiin\u0163ifice: [\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.9.6″ background_color=”rgba(117,194,33,0.23)” background_image=”https:\/\/pdi.ro\/2021\/wp-content\/uploads\/2021\/05\/PDI2021_header_1920x550px.jpg” background_blend=”multiply” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”97px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#75c221″ bottom_divider_flip=”vertical” bottom_divider_height_tablet=”” bottom_divider_height_phone=”59px” bottom_divider_height_last_edited=”on|desktop” saved_tabs=”all” global_module=”9177″][et_pb_row column_structure=”3_5,2_5″ _builder_version=”3.25″ 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A. Panu nr. 13, Iasi<\/span> [\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.0.11″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off”]<\/p>\n Parteneri Media<\/p>\n [\/et_pb_text][et_pb_gallery gallery_ids=”7203,7204,9252,9464,10156,10157″ fullwidth=”on” _builder_version=”4.9.7″ transform_scale=”86%|86%” max_width=”63%” auto=”on” auto_speed=”3500″ hover_transition_duration=”100ms” hover_transition_delay=”50ms” hover_transition_speed_curve=”ease-in-out” locked=”off”][\/et_pb_gallery][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" \u015eef lucr. Dr. B\u0103descu Aida Corina Universitatea de Medicin\u0103 \u015fi Farmacie “Grigore T. Popa”, Ia\u015fiDiagnosticul molecular \u00een infec\u021bia SARS-CoV-2, de la perfec\u021biunea tehnicii la arta interpret\u0103rii rezultatelor Autor: Aida Corina B\u0103descu Cuvinte cheie: virus SARS-CoV-2, diagnostic de laborator, metode moleculare Sopul acestei prezent\u0103ri este acela de a oferi participan\u021bilor o privire de ansamblu asupra posibilit\u0103\u021bilor […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-8649","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8649","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/comments?post=8649"}],"version-history":[{"count":5,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8649\/revisions"}],"predecessor-version":[{"id":10553,"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/pages\/8649\/revisions\/10553"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2021\/wp-json\/wp\/v2\/media?parent=8649"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Autor: Aida Corina B\u0103descu
<\/span><\/p>\n
The infection with the SARS-CoV-2 virus represented an huge challenge for the romanian medicine also from the point of view of the etiological diagnosis possibilities. Molecular diagnostic techniques were routinely used in our country only for the diagnosis and monitoring of viral infections that received specific therapy (HIV infection, viral hepatitis) or for the diagnosis of tuberculosis. The rapid expansion of molecular diagnostic capabilities was the biggest challenge for laboratory medicine, at the beginning of the pandemic being necessary both the development of technical means and specific training of staff to perform these tests that require a higher level of training of laboratory staff.
Modern diagnostic methods (hybridization, gene amplification \u2013 PCR, sequencing), based on the detection of genes specific to this pathogen, provide the laboratory and the clinician with the most valuable tool for rapid, very sensitive and very specific detection of the virus but also allow a quantitative or semi-quantitative assessment of its level in respiratory secretions, with a major impact on the rate of transmission to contacts. The inherent appearance of virus mutations, favored by the very large number of infected people globally, has also generated the need to track these mutations and emerging viral variants, also through molecular techniques of PCR or genomic sequencing.
The presentation will include a review of the molecular diagnostic tests used in the diagnosis of SARS-CoV-2 virus infection, with their advantages and disadvantages but also with the interpretation problems they raise.<\/p>\n
Disciplina de Microbiologie
Facultatea de Medicin\u0103
U.M.F. \u201dGrigore T. Popa\u201d Ia\u015fi<\/p>\n
Doctor \u00een \u015ftiin\u0163e medicale \u2013 specialitatea Dermatovenerologie.
Medic Coordonator Laboratorul de Biologie Molecular\u0103, Spitalul de Boli Infec\u0163ioase “Sf. Parascheva\u201d Ia\u015fi<\/p>\n
\u2022 European Society for Clinical Microbiology and Infectious Disease
\u2022 Societatea Rom\u00e2n\u0103 de Microbiologie
\u2022 Societatea Rom\u00e2n\u0103 de Imunologie
\u2022 Societatea Rom\u00e2n\u0103 de Micologie<\/p>\n<\/span><\/p>\n
Tel.: 0332.40.88.00-05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n