<\/span><\/strong><\/h4>\n<\/span><\/strong><\/h4>\nCercet\u0103tor \u0218tii\u021bific gr. I Constantin Carolina <\/span><\/strong>
<\/span><\/strong><\/h3>\nInstitutul Na\u0163ional de Cercetare-Dezvoltare \u00een Domeniul Patologiei \u015fi \u015etiin\u0163elor Biomedicale “Victor Babe\u015f\u201d Bucure\u015fti
Serviciul de Anatomie Patologic\u0103, Spitalul Clinic Colentina, Bucure\u015fti<\/span><\/strong><\/p>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.20.0″ global_colors_info=”{}”]<\/p>\n
Modific\u0103ri fenotipice ale celulelor NK – noua abordare \u00een imunoterapia melanomului<\/span><\/p>\nAutori: Carolina Constantin1,2, Gheorghi\u021ba Isvoranu1, Mihaela Surcel1, Adriana Narcisa Munteanu1, Sabina Zurac 2,3, Monica Neagu1,2,4<\/p>\n
1 Laborator Imunologie, Institutul Na\u021bional de Patologie \u201eVictor Babe\u0219\u201d Bucure\u0219ti, Rom\u00e2nia
2 Departament Patologie, Spital Clinic Colentina, Bucure\u0219ti, Rom\u00e2nia
3 \u0218coala Doctoral\u0103, Facultatea de Biologie, Universitatea Bucure\u0219ti, Rom\u00e2nia<\/p>\n
Cuvinte cheie: celule NK, fenotip imun, terapie, melanom<\/p>\n
Introducere. Imunoterapia devine un reper extrem de actual \u00een cancer iar celulele imune reprezint\u0103 principalul instrument terapeutic \u00een acest demers. Activitatea citolitic\u0103 a celulelor natural uciga\u0219e (natural killer NK) poate fi explorat\u0103 ca o nou\u0103 cale \u00een imunoterapia anti-tumoral\u0103. De\u0219i activarea ini\u021bial\u0103 a acestora in vitro de citokine precum IL-2, IL-12, IL-15, sau IL-18 a fost studiat\u0103 intens, efectele biologice ale activ\u0103rii NK \u00een contextul interac\u021biei cu celulele tumorale r\u0103m\u00e2ne un obiectiv insuficient abordat.
Materiale \u0219i metod\u0103. S-au investigat prin citometrie de flux modific\u0103rile de fenotip ale celulelor NK murine, \u00een sisteme experimentale de co-cultivare cu celule tumorale din linia standard de melanom B16F10.
Rezultate: A fost observat\u0103 supraexpresia unor markeri de activare NK, respectiv CD69, CD132 \u0219i CD25, expresie asociat\u0103 cu capacit\u0103\u021bi citotoxice \u0219i imunomodulatoare crescute. \u00cen plus, \u00een urma activ\u0103rii tumorale a celulelor NK a fost \u00eenregistrat\u0103 reglarea negativ\u0103 a receptorului NKp46 \u0219i a unor molecule de maturare cum sunt CD49b \u0219i CD122.
Concluzii. Acest tip de date poate contribui la definirea unui fenotip NK modulat de celulele tumorale, independent de stimularea via citokine, oferind noi dimensiuni r\u0103spunsurilor anti-tumorale NK, acceler\u00e2nd poten\u021bialul terapeutic al acestora \u00een cancer, respectiv \u00een melanom.
Studiu finan\u021bat de proiectele: PN-III-P4-PCE-2021-0549; Program Nucleu 2022-2026 MEMOGEN: PN 23.16.01.03\/2022; COST CA21108 European Network for Skin Engineering and Modeling (NETSKINMODELS).<\/p>\n
NK cells phenotypic changes in tumor setting – next level in melanoma immunotherapy<\/span><\/p>\nAuthors: Carolina Constantin1,2, Gheorghi\u021ba Isvoranu1, Mihaela Surcel1, Adriana Narcisa Munteanu1, Sabina Zurac 2, Monica Neagu1,2,3<\/p>\n
1 Immunology Laboratory, \u201eVictor Babe\u0219\u201d National Institute of Pathology, Bucharest, Romania
2 Pathology Department, Colentina Clinical Hospital, Bucharest, Romania
3 Doctoral School, Faculty of Biology, University of Bucharest, Romania<\/p>\n
Keywords: NK cells, immune phenotype, therapy, melanoma<\/p>\n
Introduction. Immunotherapy emerges as a current landmark in cancer, where immune cells represent the main therapeutic tools in this attempt. Thus, the cytolytic activity of natural killer (NK) cells may be explored as another path for anti-tumor treatment. Although in vitro \u201cpriming\u201d of NK cells by cytokines such as IL-2, IL-12, IL-15, or IL-18 has been extensively studied, the biological consequences of NK cell activation in response to tumor cell interactions have not been yet in depth examined.
Material and method. Our aim was to investigate by flow-cytometry the potential phenotype changes of murine NK cells upon co-incubation with melanoma B16F10 cell line.
Results. Upregulation of some NK cell activation markers was noticed, such as CD69, CD132 and CD25 associated with enhanced NK cell cytotoxicity and immunomodulatory functions. In addition, the downregulation of activation receptor NKp46 and other maturation molecules like CD49b and CD122 following tumor-priming was observed.
Conclusion. These findings might contribute to define the NK cells phenotype following their encounters with tumor cells, independent of cytokine stimulation, and provide insight into tumor-specific NK cell responses accelerating the therapeutic potential of NK cells in cancer, respectively in melanoma.
This work was supported by following grants: PN-III-P4-PCE-2021-0549; The Core Program 2022-2026 MEMOGEN: PN 23.16.01.03\/2022; COST Action CA21108 European Network for Skin Engineering and Modeling (NETSKINMODELS).<\/p>\n
Scurt CV<\/span><\/p>\nCarolina CONSTANTIN, Senior researcher I, PhD, biochemist, \u201cVictor Babe\u0219\u201d National Institute of Pathology, Immunology Laboratory & Colentina Clinical Hospital, Research Core of Pathology Department, Bucharest. Research topics: immune parameters analysis and biomarker discovery in melanoma and non-melanoma skin cancer; co-inventor for 4 patents related to photodynamic therapy PDT in skin pathologies. Dr. Constantin conducted national research projects linked to novel photosensitizers for cancer PDT, drug-delivery nanosystems for skin regeneration; partner responsible for MNT_ERANET project 7030-1\/2010 related to novel porphyrins for theranostics.
Specialization in Molecular Biology at Ludwig Institute for Cancer Research, Signal Transduction Unit (Bruxelles); Cellular Biology specialization at University of Athens, Biochemistry and Physiology Department, in the frame of NATO SfP 982838\/2007 project. Technical expert in 5 EU projects for biomedical assays; teaching experience in protein microarray technology; volunteer reviewer for several ISI journals. Currently involved as MC member for COST Action CA18127 – International Nucleome Consortium (INC); MC substitute of the management committee for COST action CA16120 – European Epitranscriptomics Network (EPITRAN); alternate member of the management committee for CA18125 – Advanced Engineering and Research of Aerogels for Environment and Life Sciences (AEROGELS); membership: CA21108 European Network for Skin Engineering and Modeling (NETSKINMODELS).
Reviewer for the following Journals: Cancer Management and Research; Genes; OncoTargets and Therapy; International Journal of Nanomedicine; Nanomedicine; International Journal of General Medicine (Dove Press); Food and Chemical Toxicology (Elsevier); Evidence-Based Complementary and Alternative Medicine (Hindawi); Environmental Toxicology and Pharmacology; Seminars in Cancers; Theranostics; Frontiers in Oncology; Frontiers in Bioscience-Landmark; Journal of Pharmaceutical Research International.
Editorial Board membership: Journal of Personalized Medicine (since 2022); Revista de Chimie (Bucharest) (since 2020).<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.19.4″ background_color=”rgba(240,98,42,0.19)” background_image=”https:\/\/pdi.ro\/2023\/wp-content\/uploads\/2022\/12\/PDI2023_header__-scaled.jpg” background_blend=”multiply” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”97px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#F0622A” bottom_divider_flip=”vertical” bottom_divider_height_tablet=”” bottom_divider_height_phone=”59px” bottom_divider_height_last_edited=”on|desktop” global_module=”9177″ saved_tabs=”all” global_colors_info=”{}”][et_pb_row column_structure=”3_5,2_5″ _builder_version=”4.19.4″ custom_padding=”100px|0px|69px|0px|false|false” global_colors_info=”{}”][et_pb_column type=”3_5″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text disabled_on=”off|off|off” _builder_version=”4.20.2″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” text_line_height=”1.2em” header_font=”||||||||” background_layout=”dark” text_font_size_tablet=”” text_font_size_phone=”” text_font_size_last_edited=”on|desktop” global_colors_info=”{}”]<\/p>\n
Contacteaz\u0103 operatorul PDI 2023<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@pdi.ro” submit_button_text=”Trimite mesajul” disabled_on=”off|off|off” _builder_version=”4.20.2″ _unique_id=”794aaef5-e005-432a-99fb-9ca53e06d67b” title_font=”||||||||” custom_button=”on” button_text_color=”#F0622A” global_colors_info=”{}”][et_pb_contact_field field_id=”Name” field_title=”Nume” _builder_version=”4.16″ global_colors_info=”{}” button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”Adresa de e-mail” field_type=”email” _builder_version=”4.16″ global_colors_info=”{}” button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Mesajul dvs.” field_type=”text” fullwidth_field=”on” _builder_version=”4.16″ global_colors_info=”{}” button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.19.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” global_colors_info=”{}”]<\/p>\n
Operatorul PDI 2023<\/p>\n
[\/et_pb_text][et_pb_text _builder_version=”4.16″ text_font=”||||||||” global_colors_info=”{}”]<\/p>\n
![\"\"](\"https:\/\/pdi.ro\/2023\/2022\/wp-content\/uploads\/2020\/02\/eventer_logo_150px.png\")
<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAdresa: Str. A. Panu nr. 13, Iasi<\/span>
Tel.: 0332.40.88.00-05<\/span>
E-mail: contact@pdi.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.20.2″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off” global_colors_info=”{}”]<\/p>\n
Parteneri Media<\/p>\n
[\/et_pb_text][et_pb_slider disabled_on=”off|off|off” _builder_version=”4.20.4″ _module_preset=”default” position_origin_r=”bottom_left” width=”200px” max_width=”200px” content_width=”200px” content_max_width=”200px” min_height=”100px” height=”100px” max_height=”100px” custom_margin=”0px||0px||false|false” custom_padding=”0px||0px||false|false” auto=”on” auto_speed=”3000″ global_colors_info=”{}”][et_pb_slide _builder_version=”4.20.2″ _module_preset=”default” background_enable_color=”off” background_image=”https:\/\/pdi.ro\/2023\/2022\/wp-content\/uploads\/2022\/05\/timpul_.jpg” background_enable_image=”on” background_size=”initial” custom_padding=”0px||0px||false|false” link_option_url=”#” link_option_url_new_window=”on” global_colors_info=”{}” sticky_transition=”on”][\/et_pb_slide][et_pb_slide _builder_version=”4.20.2″ _module_preset=”default” background_enable_color=”off” background_image=”https:\/\/pdi.ro\/2023\/2022\/wp-content\/uploads\/2022\/05\/medical-market_.jpg” background_enable_image=”on” background_size=”initial” custom_padding=”0px||0px||false|false” link_option_url=”https:\/\/issuu.com\/finwatch\/docs\/dermato?fr=sYWIzMjQ1MDUxNTY” link_option_url_new_window=”on” global_colors_info=”{}” sticky_transition=”on”][\/et_pb_slide][et_pb_slide button_text=”Click Here” _builder_version=”4.20.4″ _module_preset=”default” background_color=”RGBA(255,255,255,0)” background_enable_color=”on” background_image=”https:\/\/pdi.ro\/2023\/wp-content\/uploads\/2023\/04\/Logo-JURMED-RGB.jpg” background_enable_image=”on” background_size=”contain” link_option_url=”https:\/\/jurmed.ro\/” link_option_url_new_window=”on” global_colors_info=”{}” sticky_transition=”on”][\/et_pb_slide][\/et_pb_slider][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"
Cercet\u0103tor \u0218tii\u021bific gr. I Constantin Carolina Institutul Na\u0163ional de Cercetare-Dezvoltare \u00een Domeniul Patologiei \u015fi \u015etiin\u0163elor Biomedicale “Victor Babe\u015f\u201d Bucure\u015ftiServiciul de Anatomie Patologic\u0103, Spitalul Clinic Colentina, Bucure\u015ftiModific\u0103ri fenotipice ale celulelor NK – noua abordare \u00een imunoterapia melanomului Autori: Carolina Constantin1,2, Gheorghi\u021ba Isvoranu1, Mihaela Surcel1, Adriana Narcisa Munteanu1, Sabina Zurac 2,3, Monica Neagu1,2,4 1 Laborator Imunologie, Institutul […]<\/p>\n","protected":false},"author":2,"featured_media":11323,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[12],"tags":[],"class_list":["post-7646","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lectori"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/posts\/7646","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/comments?post=7646"}],"version-history":[{"count":26,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/posts\/7646\/revisions"}],"predecessor-version":[{"id":13153,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/posts\/7646\/revisions\/13153"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/media\/11323"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/media?parent=7646"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/categories?post=7646"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/tags?post=7646"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Cercet\u0103tor \u0218tii\u021bific gr. I Constantin Carolina <\/span><\/strong>
<\/span><\/strong><\/h3>\nInstitutul Na\u0163ional de Cercetare-Dezvoltare \u00een Domeniul Patologiei \u015fi \u015etiin\u0163elor Biomedicale “Victor Babe\u015f\u201d Bucure\u015fti
Serviciul de Anatomie Patologic\u0103, Spitalul Clinic Colentina, Bucure\u015fti<\/span><\/strong><\/p>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.20.0″ global_colors_info=”{}”]<\/p>\n
Modific\u0103ri fenotipice ale celulelor NK – noua abordare \u00een imunoterapia melanomului<\/span><\/p>\nAutori: Carolina Constantin1,2, Gheorghi\u021ba Isvoranu1, Mihaela Surcel1, Adriana Narcisa Munteanu1, Sabina Zurac 2,3, Monica Neagu1,2,4<\/p>\n
1 Laborator Imunologie, Institutul Na\u021bional de Patologie \u201eVictor Babe\u0219\u201d Bucure\u0219ti, Rom\u00e2nia
2 Departament Patologie, Spital Clinic Colentina, Bucure\u0219ti, Rom\u00e2nia
3 \u0218coala Doctoral\u0103, Facultatea de Biologie, Universitatea Bucure\u0219ti, Rom\u00e2nia<\/p>\n
Cuvinte cheie: celule NK, fenotip imun, terapie, melanom<\/p>\n
Introducere. Imunoterapia devine un reper extrem de actual \u00een cancer iar celulele imune reprezint\u0103 principalul instrument terapeutic \u00een acest demers. Activitatea citolitic\u0103 a celulelor natural uciga\u0219e (natural killer NK) poate fi explorat\u0103 ca o nou\u0103 cale \u00een imunoterapia anti-tumoral\u0103. De\u0219i activarea ini\u021bial\u0103 a acestora in vitro de citokine precum IL-2, IL-12, IL-15, sau IL-18 a fost studiat\u0103 intens, efectele biologice ale activ\u0103rii NK \u00een contextul interac\u021biei cu celulele tumorale r\u0103m\u00e2ne un obiectiv insuficient abordat.
Materiale \u0219i metod\u0103. S-au investigat prin citometrie de flux modific\u0103rile de fenotip ale celulelor NK murine, \u00een sisteme experimentale de co-cultivare cu celule tumorale din linia standard de melanom B16F10.
Rezultate: A fost observat\u0103 supraexpresia unor markeri de activare NK, respectiv CD69, CD132 \u0219i CD25, expresie asociat\u0103 cu capacit\u0103\u021bi citotoxice \u0219i imunomodulatoare crescute. \u00cen plus, \u00een urma activ\u0103rii tumorale a celulelor NK a fost \u00eenregistrat\u0103 reglarea negativ\u0103 a receptorului NKp46 \u0219i a unor molecule de maturare cum sunt CD49b \u0219i CD122.
Concluzii. Acest tip de date poate contribui la definirea unui fenotip NK modulat de celulele tumorale, independent de stimularea via citokine, oferind noi dimensiuni r\u0103spunsurilor anti-tumorale NK, acceler\u00e2nd poten\u021bialul terapeutic al acestora \u00een cancer, respectiv \u00een melanom.
Studiu finan\u021bat de proiectele: PN-III-P4-PCE-2021-0549; Program Nucleu 2022-2026 MEMOGEN: PN 23.16.01.03\/2022; COST CA21108 European Network for Skin Engineering and Modeling (NETSKINMODELS).<\/p>\n
NK cells phenotypic changes in tumor setting – next level in melanoma immunotherapy<\/span><\/p>\nAuthors: Carolina Constantin1,2, Gheorghi\u021ba Isvoranu1, Mihaela Surcel1, Adriana Narcisa Munteanu1, Sabina Zurac 2, Monica Neagu1,2,3<\/p>\n
1 Immunology Laboratory, \u201eVictor Babe\u0219\u201d National Institute of Pathology, Bucharest, Romania
2 Pathology Department, Colentina Clinical Hospital, Bucharest, Romania
3 Doctoral School, Faculty of Biology, University of Bucharest, Romania<\/p>\n
Keywords: NK cells, immune phenotype, therapy, melanoma<\/p>\n
Introduction. Immunotherapy emerges as a current landmark in cancer, where immune cells represent the main therapeutic tools in this attempt. Thus, the cytolytic activity of natural killer (NK) cells may be explored as another path for anti-tumor treatment. Although in vitro \u201cpriming\u201d of NK cells by cytokines such as IL-2, IL-12, IL-15, or IL-18 has been extensively studied, the biological consequences of NK cell activation in response to tumor cell interactions have not been yet in depth examined.
Material and method. Our aim was to investigate by flow-cytometry the potential phenotype changes of murine NK cells upon co-incubation with melanoma B16F10 cell line.
Results. Upregulation of some NK cell activation markers was noticed, such as CD69, CD132 and CD25 associated with enhanced NK cell cytotoxicity and immunomodulatory functions. In addition, the downregulation of activation receptor NKp46 and other maturation molecules like CD49b and CD122 following tumor-priming was observed.
Conclusion. These findings might contribute to define the NK cells phenotype following their encounters with tumor cells, independent of cytokine stimulation, and provide insight into tumor-specific NK cell responses accelerating the therapeutic potential of NK cells in cancer, respectively in melanoma.
This work was supported by following grants: PN-III-P4-PCE-2021-0549; The Core Program 2022-2026 MEMOGEN: PN 23.16.01.03\/2022; COST Action CA21108 European Network for Skin Engineering and Modeling (NETSKINMODELS).<\/p>\n
Scurt CV<\/span><\/p>\nCarolina CONSTANTIN, Senior researcher I, PhD, biochemist, \u201cVictor Babe\u0219\u201d National Institute of Pathology, Immunology Laboratory & Colentina Clinical Hospital, Research Core of Pathology Department, Bucharest. Research topics: immune parameters analysis and biomarker discovery in melanoma and non-melanoma skin cancer; co-inventor for 4 patents related to photodynamic therapy PDT in skin pathologies. Dr. Constantin conducted national research projects linked to novel photosensitizers for cancer PDT, drug-delivery nanosystems for skin regeneration; partner responsible for MNT_ERANET project 7030-1\/2010 related to novel porphyrins for theranostics.
Specialization in Molecular Biology at Ludwig Institute for Cancer Research, Signal Transduction Unit (Bruxelles); Cellular Biology specialization at University of Athens, Biochemistry and Physiology Department, in the frame of NATO SfP 982838\/2007 project. Technical expert in 5 EU projects for biomedical assays; teaching experience in protein microarray technology; volunteer reviewer for several ISI journals. Currently involved as MC member for COST Action CA18127 – International Nucleome Consortium (INC); MC substitute of the management committee for COST action CA16120 – European Epitranscriptomics Network (EPITRAN); alternate member of the management committee for CA18125 – Advanced Engineering and Research of Aerogels for Environment and Life Sciences (AEROGELS); membership: CA21108 European Network for Skin Engineering and Modeling (NETSKINMODELS).
Reviewer for the following Journals: Cancer Management and Research; Genes; OncoTargets and Therapy; International Journal of Nanomedicine; Nanomedicine; International Journal of General Medicine (Dove Press); Food and Chemical Toxicology (Elsevier); Evidence-Based Complementary and Alternative Medicine (Hindawi); Environmental Toxicology and Pharmacology; Seminars in Cancers; Theranostics; Frontiers in Oncology; Frontiers in Bioscience-Landmark; Journal of Pharmaceutical Research International.
Editorial Board membership: Journal of Personalized Medicine (since 2022); Revista de Chimie (Bucharest) (since 2020).<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.19.4″ background_color=”rgba(240,98,42,0.19)” background_image=”https:\/\/pdi.ro\/2023\/wp-content\/uploads\/2022\/12\/PDI2023_header__-scaled.jpg” background_blend=”multiply” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”97px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#F0622A” bottom_divider_flip=”vertical” bottom_divider_height_tablet=”” bottom_divider_height_phone=”59px” bottom_divider_height_last_edited=”on|desktop” global_module=”9177″ saved_tabs=”all” global_colors_info=”{}”][et_pb_row column_structure=”3_5,2_5″ _builder_version=”4.19.4″ custom_padding=”100px|0px|69px|0px|false|false” global_colors_info=”{}”][et_pb_column type=”3_5″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text disabled_on=”off|off|off” _builder_version=”4.20.2″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” text_line_height=”1.2em” header_font=”||||||||” background_layout=”dark” text_font_size_tablet=”” text_font_size_phone=”” text_font_size_last_edited=”on|desktop” global_colors_info=”{}”]<\/p>\n
Contacteaz\u0103 operatorul PDI 2023<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@pdi.ro” submit_button_text=”Trimite mesajul” disabled_on=”off|off|off” _builder_version=”4.20.2″ _unique_id=”794aaef5-e005-432a-99fb-9ca53e06d67b” title_font=”||||||||” custom_button=”on” button_text_color=”#F0622A” global_colors_info=”{}”][et_pb_contact_field field_id=”Name” field_title=”Nume” _builder_version=”4.16″ global_colors_info=”{}” button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”Adresa de e-mail” field_type=”email” _builder_version=”4.16″ global_colors_info=”{}” button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Mesajul dvs.” field_type=”text” fullwidth_field=”on” _builder_version=”4.16″ global_colors_info=”{}” button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.19.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” global_colors_info=”{}”]<\/p>\n
Operatorul PDI 2023<\/p>\n
[\/et_pb_text][et_pb_text _builder_version=”4.16″ text_font=”||||||||” global_colors_info=”{}”]<\/p>\n
<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAdresa: Str. A. Panu nr. 13, Iasi<\/span>
Tel.: 0332.40.88.00-05<\/span>
E-mail: contact@pdi.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.20.2″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off” global_colors_info=”{}”]<\/p>\n
Parteneri Media<\/p>\n
[\/et_pb_text][et_pb_slider disabled_on=”off|off|off” _builder_version=”4.20.4″ _module_preset=”default” position_origin_r=”bottom_left” width=”200px” max_width=”200px” content_width=”200px” content_max_width=”200px” min_height=”100px” height=”100px” max_height=”100px” custom_margin=”0px||0px||false|false” custom_padding=”0px||0px||false|false” auto=”on” auto_speed=”3000″ global_colors_info=”{}”][et_pb_slide _builder_version=”4.20.2″ _module_preset=”default” background_enable_color=”off” background_image=”https:\/\/pdi.ro\/2023\/2022\/wp-content\/uploads\/2022\/05\/timpul_.jpg” background_enable_image=”on” background_size=”initial” custom_padding=”0px||0px||false|false” link_option_url=”#” link_option_url_new_window=”on” global_colors_info=”{}” sticky_transition=”on”][\/et_pb_slide][et_pb_slide _builder_version=”4.20.2″ _module_preset=”default” background_enable_color=”off” background_image=”https:\/\/pdi.ro\/2023\/2022\/wp-content\/uploads\/2022\/05\/medical-market_.jpg” background_enable_image=”on” background_size=”initial” custom_padding=”0px||0px||false|false” link_option_url=”https:\/\/issuu.com\/finwatch\/docs\/dermato?fr=sYWIzMjQ1MDUxNTY” link_option_url_new_window=”on” global_colors_info=”{}” sticky_transition=”on”][\/et_pb_slide][et_pb_slide button_text=”Click Here” _builder_version=”4.20.4″ _module_preset=”default” background_color=”RGBA(255,255,255,0)” background_enable_color=”on” background_image=”https:\/\/pdi.ro\/2023\/wp-content\/uploads\/2023\/04\/Logo-JURMED-RGB.jpg” background_enable_image=”on” background_size=”contain” link_option_url=”https:\/\/jurmed.ro\/” link_option_url_new_window=”on” global_colors_info=”{}” sticky_transition=”on”][\/et_pb_slide][\/et_pb_slider][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"
Cercet\u0103tor \u0218tii\u021bific gr. I Constantin Carolina Institutul Na\u0163ional de Cercetare-Dezvoltare \u00een Domeniul Patologiei \u015fi \u015etiin\u0163elor Biomedicale “Victor Babe\u015f\u201d Bucure\u015ftiServiciul de Anatomie Patologic\u0103, Spitalul Clinic Colentina, Bucure\u015ftiModific\u0103ri fenotipice ale celulelor NK – noua abordare \u00een imunoterapia melanomului Autori: Carolina Constantin1,2, Gheorghi\u021ba Isvoranu1, Mihaela Surcel1, Adriana Narcisa Munteanu1, Sabina Zurac 2,3, Monica Neagu1,2,4 1 Laborator Imunologie, Institutul […]<\/p>\n","protected":false},"author":2,"featured_media":11323,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[12],"tags":[],"class_list":["post-7646","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lectori"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/posts\/7646","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/comments?post=7646"}],"version-history":[{"count":26,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/posts\/7646\/revisions"}],"predecessor-version":[{"id":13153,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/posts\/7646\/revisions\/13153"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/media\/11323"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/media?parent=7646"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/categories?post=7646"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/tags?post=7646"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Serviciul de Anatomie Patologic\u0103, Spitalul Clinic Colentina, Bucure\u015fti<\/span><\/strong><\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.20.0″ global_colors_info=”{}”]<\/p>\n
Modific\u0103ri fenotipice ale celulelor NK – noua abordare \u00een imunoterapia melanomului<\/span><\/p>\n Autori: Carolina Constantin1,2, Gheorghi\u021ba Isvoranu1, Mihaela Surcel1, Adriana Narcisa Munteanu1, Sabina Zurac 2,3, Monica Neagu1,2,4<\/p>\n 1 Laborator Imunologie, Institutul Na\u021bional de Patologie \u201eVictor Babe\u0219\u201d Bucure\u0219ti, Rom\u00e2nia Cuvinte cheie: celule NK, fenotip imun, terapie, melanom<\/p>\n Introducere. Imunoterapia devine un reper extrem de actual \u00een cancer iar celulele imune reprezint\u0103 principalul instrument terapeutic \u00een acest demers. Activitatea citolitic\u0103 a celulelor natural uciga\u0219e (natural killer NK) poate fi explorat\u0103 ca o nou\u0103 cale \u00een imunoterapia anti-tumoral\u0103. De\u0219i activarea ini\u021bial\u0103 a acestora in vitro de citokine precum IL-2, IL-12, IL-15, sau IL-18 a fost studiat\u0103 intens, efectele biologice ale activ\u0103rii NK \u00een contextul interac\u021biei cu celulele tumorale r\u0103m\u00e2ne un obiectiv insuficient abordat. NK cells phenotypic changes in tumor setting – next level in melanoma immunotherapy<\/span><\/p>\n Authors: Carolina Constantin1,2, Gheorghi\u021ba Isvoranu1, Mihaela Surcel1, Adriana Narcisa Munteanu1, Sabina Zurac 2, Monica Neagu1,2,3<\/p>\n 1 Immunology Laboratory, \u201eVictor Babe\u0219\u201d National Institute of Pathology, Bucharest, Romania Keywords: NK cells, immune phenotype, therapy, melanoma<\/p>\n Introduction. Immunotherapy emerges as a current landmark in cancer, where immune cells represent the main therapeutic tools in this attempt. Thus, the cytolytic activity of natural killer (NK) cells may be explored as another path for anti-tumor treatment. Although in vitro \u201cpriming\u201d of NK cells by cytokines such as IL-2, IL-12, IL-15, or IL-18 has been extensively studied, the biological consequences of NK cell activation in response to tumor cell interactions have not been yet in depth examined. Scurt CV<\/span><\/p>\n Carolina CONSTANTIN, Senior researcher I, PhD, biochemist, \u201cVictor Babe\u0219\u201d National Institute of Pathology, Immunology Laboratory & Colentina Clinical Hospital, Research Core of Pathology Department, Bucharest. Research topics: immune parameters analysis and biomarker discovery in melanoma and non-melanoma skin cancer; co-inventor for 4 patents related to photodynamic therapy PDT in skin pathologies. Dr. Constantin conducted national research projects linked to novel photosensitizers for cancer PDT, drug-delivery nanosystems for skin regeneration; partner responsible for MNT_ERANET project 7030-1\/2010 related to novel porphyrins for theranostics. [\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.19.4″ background_color=”rgba(240,98,42,0.19)” background_image=”https:\/\/pdi.ro\/2023\/wp-content\/uploads\/2022\/12\/PDI2023_header__-scaled.jpg” background_blend=”multiply” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”97px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#F0622A” bottom_divider_flip=”vertical” bottom_divider_height_tablet=”” bottom_divider_height_phone=”59px” bottom_divider_height_last_edited=”on|desktop” global_module=”9177″ saved_tabs=”all” global_colors_info=”{}”][et_pb_row column_structure=”3_5,2_5″ _builder_version=”4.19.4″ custom_padding=”100px|0px|69px|0px|false|false” global_colors_info=”{}”][et_pb_column type=”3_5″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text disabled_on=”off|off|off” _builder_version=”4.20.2″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” text_line_height=”1.2em” header_font=”||||||||” background_layout=”dark” text_font_size_tablet=”” text_font_size_phone=”” text_font_size_last_edited=”on|desktop” global_colors_info=”{}”]<\/p>\n Contacteaz\u0103 operatorul PDI 2023<\/p>\n [\/et_pb_text][et_pb_contact_form email=”contact@pdi.ro” submit_button_text=”Trimite mesajul” disabled_on=”off|off|off” _builder_version=”4.20.2″ _unique_id=”794aaef5-e005-432a-99fb-9ca53e06d67b” title_font=”||||||||” custom_button=”on” button_text_color=”#F0622A” global_colors_info=”{}”][et_pb_contact_field field_id=”Name” field_title=”Nume” _builder_version=”4.16″ global_colors_info=”{}” button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”Adresa de e-mail” field_type=”email” _builder_version=”4.16″ global_colors_info=”{}” button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Mesajul dvs.” field_type=”text” fullwidth_field=”on” _builder_version=”4.16″ global_colors_info=”{}” button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.19.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” global_colors_info=”{}”]<\/p>\n Operatorul PDI 2023<\/p>\n [\/et_pb_text][et_pb_text _builder_version=”4.16″ text_font=”||||||||” global_colors_info=”{}”]<\/p>\n <\/span><\/p>\n <\/span><\/p>\n Adresa: Str. A. Panu nr. 13, Iasi<\/span> [\/et_pb_text][et_pb_text disabled_on=”off|off|off” _builder_version=”4.20.2″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” locked=”off” global_colors_info=”{}”]<\/p>\n Parteneri Media<\/p>\n [\/et_pb_text][et_pb_slider disabled_on=”off|off|off” _builder_version=”4.20.4″ _module_preset=”default” position_origin_r=”bottom_left” width=”200px” max_width=”200px” content_width=”200px” content_max_width=”200px” min_height=”100px” height=”100px” max_height=”100px” custom_margin=”0px||0px||false|false” custom_padding=”0px||0px||false|false” auto=”on” auto_speed=”3000″ global_colors_info=”{}”][et_pb_slide _builder_version=”4.20.2″ _module_preset=”default” background_enable_color=”off” background_image=”https:\/\/pdi.ro\/2023\/2022\/wp-content\/uploads\/2022\/05\/timpul_.jpg” background_enable_image=”on” background_size=”initial” custom_padding=”0px||0px||false|false” link_option_url=”#” link_option_url_new_window=”on” global_colors_info=”{}” sticky_transition=”on”][\/et_pb_slide][et_pb_slide _builder_version=”4.20.2″ _module_preset=”default” background_enable_color=”off” background_image=”https:\/\/pdi.ro\/2023\/2022\/wp-content\/uploads\/2022\/05\/medical-market_.jpg” background_enable_image=”on” background_size=”initial” custom_padding=”0px||0px||false|false” link_option_url=”https:\/\/issuu.com\/finwatch\/docs\/dermato?fr=sYWIzMjQ1MDUxNTY” link_option_url_new_window=”on” global_colors_info=”{}” sticky_transition=”on”][\/et_pb_slide][et_pb_slide button_text=”Click Here” _builder_version=”4.20.4″ _module_preset=”default” background_color=”RGBA(255,255,255,0)” background_enable_color=”on” background_image=”https:\/\/pdi.ro\/2023\/wp-content\/uploads\/2023\/04\/Logo-JURMED-RGB.jpg” background_enable_image=”on” background_size=”contain” link_option_url=”https:\/\/jurmed.ro\/” link_option_url_new_window=”on” global_colors_info=”{}” sticky_transition=”on”][\/et_pb_slide][\/et_pb_slider][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" Cercet\u0103tor \u0218tii\u021bific gr. I Constantin Carolina Institutul Na\u0163ional de Cercetare-Dezvoltare \u00een Domeniul Patologiei \u015fi \u015etiin\u0163elor Biomedicale “Victor Babe\u015f\u201d Bucure\u015ftiServiciul de Anatomie Patologic\u0103, Spitalul Clinic Colentina, Bucure\u015ftiModific\u0103ri fenotipice ale celulelor NK – noua abordare \u00een imunoterapia melanomului Autori: Carolina Constantin1,2, Gheorghi\u021ba Isvoranu1, Mihaela Surcel1, Adriana Narcisa Munteanu1, Sabina Zurac 2,3, Monica Neagu1,2,4 1 Laborator Imunologie, Institutul […]<\/p>\n","protected":false},"author":2,"featured_media":11323,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[12],"tags":[],"class_list":["post-7646","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lectori"],"_links":{"self":[{"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/posts\/7646","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/comments?post=7646"}],"version-history":[{"count":26,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/posts\/7646\/revisions"}],"predecessor-version":[{"id":13153,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/posts\/7646\/revisions\/13153"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/media\/11323"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/media?parent=7646"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/categories?post=7646"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pdi.ro\/2023\/wp-json\/wp\/v2\/tags?post=7646"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
2 Departament Patologie, Spital Clinic Colentina, Bucure\u0219ti, Rom\u00e2nia
3 \u0218coala Doctoral\u0103, Facultatea de Biologie, Universitatea Bucure\u0219ti, Rom\u00e2nia<\/p>\n
Materiale \u0219i metod\u0103. S-au investigat prin citometrie de flux modific\u0103rile de fenotip ale celulelor NK murine, \u00een sisteme experimentale de co-cultivare cu celule tumorale din linia standard de melanom B16F10.
Rezultate: A fost observat\u0103 supraexpresia unor markeri de activare NK, respectiv CD69, CD132 \u0219i CD25, expresie asociat\u0103 cu capacit\u0103\u021bi citotoxice \u0219i imunomodulatoare crescute. \u00cen plus, \u00een urma activ\u0103rii tumorale a celulelor NK a fost \u00eenregistrat\u0103 reglarea negativ\u0103 a receptorului NKp46 \u0219i a unor molecule de maturare cum sunt CD49b \u0219i CD122.
Concluzii. Acest tip de date poate contribui la definirea unui fenotip NK modulat de celulele tumorale, independent de stimularea via citokine, oferind noi dimensiuni r\u0103spunsurilor anti-tumorale NK, acceler\u00e2nd poten\u021bialul terapeutic al acestora \u00een cancer, respectiv \u00een melanom.
Studiu finan\u021bat de proiectele: PN-III-P4-PCE-2021-0549; Program Nucleu 2022-2026 MEMOGEN: PN 23.16.01.03\/2022; COST CA21108 European Network for Skin Engineering and Modeling (NETSKINMODELS).<\/p>\n
2 Pathology Department, Colentina Clinical Hospital, Bucharest, Romania
3 Doctoral School, Faculty of Biology, University of Bucharest, Romania<\/p>\n
Material and method. Our aim was to investigate by flow-cytometry the potential phenotype changes of murine NK cells upon co-incubation with melanoma B16F10 cell line.
Results. Upregulation of some NK cell activation markers was noticed, such as CD69, CD132 and CD25 associated with enhanced NK cell cytotoxicity and immunomodulatory functions. In addition, the downregulation of activation receptor NKp46 and other maturation molecules like CD49b and CD122 following tumor-priming was observed.
Conclusion. These findings might contribute to define the NK cells phenotype following their encounters with tumor cells, independent of cytokine stimulation, and provide insight into tumor-specific NK cell responses accelerating the therapeutic potential of NK cells in cancer, respectively in melanoma.
This work was supported by following grants: PN-III-P4-PCE-2021-0549; The Core Program 2022-2026 MEMOGEN: PN 23.16.01.03\/2022; COST Action CA21108 European Network for Skin Engineering and Modeling (NETSKINMODELS).<\/p>\n
Specialization in Molecular Biology at Ludwig Institute for Cancer Research, Signal Transduction Unit (Bruxelles); Cellular Biology specialization at University of Athens, Biochemistry and Physiology Department, in the frame of NATO SfP 982838\/2007 project. Technical expert in 5 EU projects for biomedical assays; teaching experience in protein microarray technology; volunteer reviewer for several ISI journals. Currently involved as MC member for COST Action CA18127 – International Nucleome Consortium (INC); MC substitute of the management committee for COST action CA16120 – European Epitranscriptomics Network (EPITRAN); alternate member of the management committee for CA18125 – Advanced Engineering and Research of Aerogels for Environment and Life Sciences (AEROGELS); membership: CA21108 European Network for Skin Engineering and Modeling (NETSKINMODELS).
Reviewer for the following Journals: Cancer Management and Research; Genes; OncoTargets and Therapy; International Journal of Nanomedicine; Nanomedicine; International Journal of General Medicine (Dove Press); Food and Chemical Toxicology (Elsevier); Evidence-Based Complementary and Alternative Medicine (Hindawi); Environmental Toxicology and Pharmacology; Seminars in Cancers; Theranostics; Frontiers in Oncology; Frontiers in Bioscience-Landmark; Journal of Pharmaceutical Research International.
Editorial Board membership: Journal of Personalized Medicine (since 2022); Revista de Chimie (Bucharest) (since 2020).<\/p>\n<\/span><\/p>\n
Tel.: 0332.40.88.00-05<\/span>
E-mail: contact@pdi.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n