<\/strong><\/span><\/h4>\n<\/strong><\/span><\/h4>\n<\/strong><\/span><\/h4>\nB\u0103dulici Sonia MD<\/strong><\/span>
Clinical Hospital “Colentina”, Bucharest<\/strong><\/span><\/h4>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.3.4″ hover_enabled=”0″]<\/p>\n
Genetic factors in thiomersal allergy: case studies <\/span>
Authors: Coralia Bleotu, Lilia Matei, Sonia C. B\u0103dulici, Mirela Radu, Annie Bonnevalle, Marinela Chiril\u0103<\/span><\/p>\nIntroduction: The genetic predisposition to allergic contact dermatitis is only partially known, despite numerous studies conducted over decades of research. Polymorphisms and mutations affecting glutathione-S-transferases (GST) P1, T1 and M1, as well as N-acetyltransferases (NAT) 1 and 2 could be associated with an increased risk of thiomersal allergy. In this context, the aims of the study were to evaluate the frequency of thiomersal sensitization in the Romanian population, to preliminary assess the role of genetic factors in triggering this sensitization, and to identify the medicinal products available in Romania that contain thiomersal as an excipient.
\nMaterial and methods: The present study included 160 patients who presented to the Colentina Clinical Hospital due to suspected allergic contact reactions. Patients were tested with the European Standard Battery and its additives (that include thiomersal). GST (GST-P1, T1 and M1) and NAT1 \/ 2 gene polymorphisms were analyzed using allelic PCR and Sanger sequencing, respectively. To establish the segregation ratio, the identification of allelic variants involved in the production of thiomersal susceptibility was performed using child-parent samples (three patients with positive reactions to thiomersal together with one of the parents).
\nIn addition, the composition of all medicinal products registered in Romania was reviewed.
\nResults: Out of the 160 patients tested with patch testing, 16 adult patients (11 atopic, 5 non-atopic) showed positive reactions to thiomersal. Concomitant sensitizations were also observed, especially to metals. These were more numerous in atopic patients as compared to non-atopic ones.
\nRegarding the genetic variants of GST, no changes in the enzymatic activity of GST-M1 and GST-T1 were found in two families. In contrast, in an another family (mother-daughter), homozygous deletion of the glutathione-S transferase M1 gene (GST-M1 * 0 \/ * 0) associated with loss of corresponding enzymatic activity was identified. In the same family, although the GST-P1 p.Ile105Val (heterozygous) polymorphism was identified in the mother, the daughter presented a GST-P1 p.Val105Val (homozygous) genotype. In a family represented by the father-daughter couple, both in the father (that has vitiligo) and in the daughter (that has allergic contact dermatitis by sensitization to thiomersal and fragrance mix I) the heterozygous variant of the GST-P1 Ile105Val polymorphism was present. Changes in the enzymatic activity of glutathione-S-transferase that is described in the literature in patients with vitiligo as a genetic predisposition to this condition could be considered as a possible explanation for contact sensitization to thiomersal in this familial case. The NAT1 genotype identified in all cases was the wild type NAT1 * 4 genotype. In the case of NAT2 the following polymorphisms were identified: NAT2 * 5 homozygous (341T> C; 481C> T), NAT2 * 5 \u2013 heterozygous (NAT2 * 5U) (282C> T; 341T> C; 481C> T; 590G> A) , and NAT2 * 6 homozygous \/ heterozygous (282C> T; 590G> A), respectively.
\nOf the medicinal products available in Romania, five contain thiomersal, four of which are ophthalmic drugs. The advice for patients was the contraindication of the administration of drugs containing thiomersal among excipients, as well as the avoidance of anti-inflammatory drugs from \u201cOXICAM\u201d class due to the possible cross-hypersensitivity reactions with thiomersal.
\nConclusion: The frequency of positive thiomersal reactions in our patients (10%) would justify its introduction into the Additional Baseline Series. Additional studies including a higher number of patients are needed to clarify the importance of genetic polymorphisms in thiomersal contact allergy. <\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”3.19.4″ background_color=”rgba(117,194,33,0.23)” background_image=”https:\/\/primaderma.ro\/en\/wp-content\/uploads\/2019\/01\/back_justflowers_overlay1.png” background_blend=”luminosity” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”77px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#75c221″ bottom_divider_flip=”vertical” bottom_divider_height_tablet=”” bottom_divider_height_phone=”54px” bottom_divider_height_last_edited=”on|desktop” global_module=”516″ saved_tabs=”all”][et_pb_row column_structure=”3_5,2_5″ _builder_version=”3.25″ custom_padding=”61px|0px|69px|0px|false|false”][et_pb_column type=”3_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.3.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” text_line_height_tablet=”” text_line_height_phone=”1.2em” text_line_height_last_edited=”on|desktop”]<\/p>\n
Contact PDI 2020’s PCO<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@primaderma.ro” submit_button_text=”Send” _builder_version=”3.19.4″ title_font=”||||||||”][et_pb_contact_field field_id=”Name” field_title=”Name” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”E-mail address” field_type=”email” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Message” field_type=”text” fullwidth_field=”on” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.3.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark”]<\/p>\n
PCO of PDI 2020<\/p>\n
[\/et_pb_text][et_pb_text _builder_version=”4.3.4″ text_font=”||||||||”]<\/p>\n
![\"\"](\"https:\/\/primaderma.ro\/en\/wp-content\/uploads\/2019\/01\/eventer_logo_150px.png\")
<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAddress: A. Panu St. no. 13, Iasi<\/span>
Tel.: +40 332.40.88.05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"
[et_pb_section fb_built=”1″ fullwidth=”on” disabled_on=”on|off|off” _builder_version=”3.22″ custom_margin=”||” custom_padding=”15px||29px” top_divider_style=”waves2″ top_divider_color=”#f0622a” top_divider_flip=”horizontal|vertical” bottom_divider_style=”waves2″ bottom_divider_color=”#fcdedb” bottom_divider_height=”105px” bottom_divider_flip=”vertical” collapsed=”off”][et_pb_fullwidth_header title=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF90aXRsZSIsInNldHRpbmdzIjp7ImJlZm9yZSI6IiIsImFmdGVyIjoiIn19@” text_orientation=”right” header_image_url=”https:\/\/primaderma.ro\/en\/wp-content\/uploads\/2019\/01\/Primaderma2019_logo_300px.png” disabled_on=”on|on|off” _builder_version=”3.19.4″ _dynamic_attributes=”title” title_font=”Kaushan Script||||||||” title_text_align=”right” title_text_color=”#f0622a” title_font_size=”50px” content_font=”|600||on|||||” content_text_align=”center” content_text_color=”#686766″ content_font_size=”15px” subhead_font=”||||||||” background_color=”rgba(239,145,107,0.54)” background_image=”https:\/\/primaderma.ro\/en\/wp-content\/uploads\/2019\/01\/back.jpg” background_layout=”light” custom_margin=”0px||8px” custom_padding=”40px||40px||true” content_font_size_tablet=”” content_font_size_phone=”15px” content_font_size_last_edited=”on|desktop”][\/et_pb_fullwidth_header][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”3.22″][et_pb_row column_structure=”2_5,3_5″ _builder_version=”3.25″][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/primaderma.ro\/en\/wp-content\/uploads\/2019\/02\/Badulici-Sonia.jpg” align=”center” align_tablet=”center” align_phone=”” align_last_edited=”on|desktop” _builder_version=”3.23″ max_width=”35%” border_radii=”on|100px|100px|100px|100px”][\/et_pb_image][\/et_pb_column][et_pb_column […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"_links":{"self":[{"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/pages\/5380"}],"collection":[{"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/comments?post=5380"}],"version-history":[{"count":6,"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/pages\/5380\/revisions"}],"predecessor-version":[{"id":8204,"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/pages\/5380\/revisions\/8204"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/media?parent=5380"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/strong><\/span><\/h4>\nB\u0103dulici Sonia MD<\/strong><\/span>
Clinical Hospital “Colentina”, Bucharest<\/strong><\/span><\/h4>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”3.25″][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.3.4″ hover_enabled=”0″]<\/p>\n
Genetic factors in thiomersal allergy: case studies <\/span>
Authors: Coralia Bleotu, Lilia Matei, Sonia C. B\u0103dulici, Mirela Radu, Annie Bonnevalle, Marinela Chiril\u0103<\/span><\/p>\nIntroduction: The genetic predisposition to allergic contact dermatitis is only partially known, despite numerous studies conducted over decades of research. Polymorphisms and mutations affecting glutathione-S-transferases (GST) P1, T1 and M1, as well as N-acetyltransferases (NAT) 1 and 2 could be associated with an increased risk of thiomersal allergy. In this context, the aims of the study were to evaluate the frequency of thiomersal sensitization in the Romanian population, to preliminary assess the role of genetic factors in triggering this sensitization, and to identify the medicinal products available in Romania that contain thiomersal as an excipient.
\nMaterial and methods: The present study included 160 patients who presented to the Colentina Clinical Hospital due to suspected allergic contact reactions. Patients were tested with the European Standard Battery and its additives (that include thiomersal). GST (GST-P1, T1 and M1) and NAT1 \/ 2 gene polymorphisms were analyzed using allelic PCR and Sanger sequencing, respectively. To establish the segregation ratio, the identification of allelic variants involved in the production of thiomersal susceptibility was performed using child-parent samples (three patients with positive reactions to thiomersal together with one of the parents).
\nIn addition, the composition of all medicinal products registered in Romania was reviewed.
\nResults: Out of the 160 patients tested with patch testing, 16 adult patients (11 atopic, 5 non-atopic) showed positive reactions to thiomersal. Concomitant sensitizations were also observed, especially to metals. These were more numerous in atopic patients as compared to non-atopic ones.
\nRegarding the genetic variants of GST, no changes in the enzymatic activity of GST-M1 and GST-T1 were found in two families. In contrast, in an another family (mother-daughter), homozygous deletion of the glutathione-S transferase M1 gene (GST-M1 * 0 \/ * 0) associated with loss of corresponding enzymatic activity was identified. In the same family, although the GST-P1 p.Ile105Val (heterozygous) polymorphism was identified in the mother, the daughter presented a GST-P1 p.Val105Val (homozygous) genotype. In a family represented by the father-daughter couple, both in the father (that has vitiligo) and in the daughter (that has allergic contact dermatitis by sensitization to thiomersal and fragrance mix I) the heterozygous variant of the GST-P1 Ile105Val polymorphism was present. Changes in the enzymatic activity of glutathione-S-transferase that is described in the literature in patients with vitiligo as a genetic predisposition to this condition could be considered as a possible explanation for contact sensitization to thiomersal in this familial case. The NAT1 genotype identified in all cases was the wild type NAT1 * 4 genotype. In the case of NAT2 the following polymorphisms were identified: NAT2 * 5 homozygous (341T> C; 481C> T), NAT2 * 5 \u2013 heterozygous (NAT2 * 5U) (282C> T; 341T> C; 481C> T; 590G> A) , and NAT2 * 6 homozygous \/ heterozygous (282C> T; 590G> A), respectively.
\nOf the medicinal products available in Romania, five contain thiomersal, four of which are ophthalmic drugs. The advice for patients was the contraindication of the administration of drugs containing thiomersal among excipients, as well as the avoidance of anti-inflammatory drugs from \u201cOXICAM\u201d class due to the possible cross-hypersensitivity reactions with thiomersal.
\nConclusion: The frequency of positive thiomersal reactions in our patients (10%) would justify its introduction into the Additional Baseline Series. Additional studies including a higher number of patients are needed to clarify the importance of genetic polymorphisms in thiomersal contact allergy. <\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”3.19.4″ background_color=”rgba(117,194,33,0.23)” background_image=”https:\/\/primaderma.ro\/en\/wp-content\/uploads\/2019\/01\/back_justflowers_overlay1.png” background_blend=”luminosity” custom_padding=”54px|0px|0|0px|false|false” top_divider_style=”waves2″ top_divider_color=”#ffffff” top_divider_height=”155px” top_divider_height_tablet=”” top_divider_height_phone=”77px” top_divider_height_last_edited=”on|desktop” bottom_divider_style=”waves2″ bottom_divider_color=”#75c221″ bottom_divider_flip=”vertical” bottom_divider_height_tablet=”” bottom_divider_height_phone=”54px” bottom_divider_height_last_edited=”on|desktop” global_module=”516″ saved_tabs=”all”][et_pb_row column_structure=”3_5,2_5″ _builder_version=”3.25″ custom_padding=”61px|0px|69px|0px|false|false”][et_pb_column type=”3_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.3.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark” text_line_height_tablet=”” text_line_height_phone=”1.2em” text_line_height_last_edited=”on|desktop”]<\/p>\n
Contact PDI 2020’s PCO<\/p>\n
[\/et_pb_text][et_pb_contact_form email=”contact@primaderma.ro” submit_button_text=”Send” _builder_version=”3.19.4″ title_font=”||||||||”][et_pb_contact_field field_id=”Name” field_title=”Name” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Email” field_title=”E-mail address” field_type=”email” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][et_pb_contact_field field_id=”Message” field_title=”Message” field_type=”text” fullwidth_field=”on” _builder_version=”3.19.4″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_contact_field][\/et_pb_contact_form][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.3.4″ text_font=”Kaushan Script||||||||” text_text_color=”#f0622a” text_font_size=”30px” header_font=”||||||||” background_layout=”dark”]<\/p>\n
PCO of PDI 2020<\/p>\n
[\/et_pb_text][et_pb_text _builder_version=”4.3.4″ text_font=”||||||||”]<\/p>\n
<\/span><\/p>\n<\/span><\/p>\n<\/span><\/p>\nAddress: A. Panu St. no. 13, Iasi<\/span>
Tel.: +40 332.40.88.05<\/span>
E-mail: inscrieri@primaderma.ro<\/a>
<\/span>Website:\u00a0<\/span>www.eventernet.ro<\/a><\/p>\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"
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Clinical Hospital “Colentina”, Bucharest<\/strong><\/span><\/h4>\n
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Genetic factors in thiomersal allergy: case studies <\/span> Introduction: The genetic predisposition to allergic contact dermatitis is only partially known, despite numerous studies conducted over decades of research. Polymorphisms and mutations affecting glutathione-S-transferases (GST) P1, T1 and M1, as well as N-acetyltransferases (NAT) 1 and 2 could be associated with an increased risk of thiomersal allergy. In this context, the aims of the study were to evaluate the frequency of thiomersal sensitization in the Romanian population, to preliminary assess the role of genetic factors in triggering this sensitization, and to identify the medicinal products available in Romania that contain thiomersal as an excipient. 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Panu St. no. 13, Iasi<\/span> [\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" [et_pb_section fb_built=”1″ fullwidth=”on” disabled_on=”on|off|off” _builder_version=”3.22″ custom_margin=”||” custom_padding=”15px||29px” top_divider_style=”waves2″ top_divider_color=”#f0622a” top_divider_flip=”horizontal|vertical” bottom_divider_style=”waves2″ bottom_divider_color=”#fcdedb” bottom_divider_height=”105px” bottom_divider_flip=”vertical” collapsed=”off”][et_pb_fullwidth_header title=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF90aXRsZSIsInNldHRpbmdzIjp7ImJlZm9yZSI6IiIsImFmdGVyIjoiIn19@” text_orientation=”right” header_image_url=”https:\/\/primaderma.ro\/en\/wp-content\/uploads\/2019\/01\/Primaderma2019_logo_300px.png” disabled_on=”on|on|off” _builder_version=”3.19.4″ _dynamic_attributes=”title” title_font=”Kaushan Script||||||||” title_text_align=”right” title_text_color=”#f0622a” title_font_size=”50px” content_font=”|600||on|||||” content_text_align=”center” content_text_color=”#686766″ content_font_size=”15px” subhead_font=”||||||||” background_color=”rgba(239,145,107,0.54)” background_image=”https:\/\/primaderma.ro\/en\/wp-content\/uploads\/2019\/01\/back.jpg” background_layout=”light” custom_margin=”0px||8px” custom_padding=”40px||40px||true” content_font_size_tablet=”” content_font_size_phone=”15px” content_font_size_last_edited=”on|desktop”][\/et_pb_fullwidth_header][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”3.22″][et_pb_row column_structure=”2_5,3_5″ _builder_version=”3.25″][et_pb_column type=”2_5″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/primaderma.ro\/en\/wp-content\/uploads\/2019\/02\/Badulici-Sonia.jpg” align=”center” align_tablet=”center” align_phone=”” align_last_edited=”on|desktop” _builder_version=”3.23″ max_width=”35%” border_radii=”on|100px|100px|100px|100px”][\/et_pb_image][\/et_pb_column][et_pb_column […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"_links":{"self":[{"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/pages\/5380"}],"collection":[{"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/comments?post=5380"}],"version-history":[{"count":6,"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/pages\/5380\/revisions"}],"predecessor-version":[{"id":8204,"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/pages\/5380\/revisions\/8204"}],"wp:attachment":[{"href":"https:\/\/pdi.ro\/en\/wp-json\/wp\/v2\/media?parent=5380"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Authors: Coralia Bleotu, Lilia Matei, Sonia C. B\u0103dulici, Mirela Radu, Annie Bonnevalle, Marinela Chiril\u0103<\/span><\/p>\n
\nMaterial and methods: The present study included 160 patients who presented to the Colentina Clinical Hospital due to suspected allergic contact reactions. Patients were tested with the European Standard Battery and its additives (that include thiomersal). GST (GST-P1, T1 and M1) and NAT1 \/ 2 gene polymorphisms were analyzed using allelic PCR and Sanger sequencing, respectively. To establish the segregation ratio, the identification of allelic variants involved in the production of thiomersal susceptibility was performed using child-parent samples (three patients with positive reactions to thiomersal together with one of the parents).
\nIn addition, the composition of all medicinal products registered in Romania was reviewed.
\nResults: Out of the 160 patients tested with patch testing, 16 adult patients (11 atopic, 5 non-atopic) showed positive reactions to thiomersal. Concomitant sensitizations were also observed, especially to metals. These were more numerous in atopic patients as compared to non-atopic ones.
\nRegarding the genetic variants of GST, no changes in the enzymatic activity of GST-M1 and GST-T1 were found in two families. In contrast, in an another family (mother-daughter), homozygous deletion of the glutathione-S transferase M1 gene (GST-M1 * 0 \/ * 0) associated with loss of corresponding enzymatic activity was identified. In the same family, although the GST-P1 p.Ile105Val (heterozygous) polymorphism was identified in the mother, the daughter presented a GST-P1 p.Val105Val (homozygous) genotype. In a family represented by the father-daughter couple, both in the father (that has vitiligo) and in the daughter (that has allergic contact dermatitis by sensitization to thiomersal and fragrance mix I) the heterozygous variant of the GST-P1 Ile105Val polymorphism was present. Changes in the enzymatic activity of glutathione-S-transferase that is described in the literature in patients with vitiligo as a genetic predisposition to this condition could be considered as a possible explanation for contact sensitization to thiomersal in this familial case. The NAT1 genotype identified in all cases was the wild type NAT1 * 4 genotype. In the case of NAT2 the following polymorphisms were identified: NAT2 * 5 homozygous (341T> C; 481C> T), NAT2 * 5 \u2013 heterozygous (NAT2 * 5U) (282C> T; 341T> C; 481C> T; 590G> A) , and NAT2 * 6 homozygous \/ heterozygous (282C> T; 590G> A), respectively.
\nOf the medicinal products available in Romania, five contain thiomersal, four of which are ophthalmic drugs. The advice for patients was the contraindication of the administration of drugs containing thiomersal among excipients, as well as the avoidance of anti-inflammatory drugs from \u201cOXICAM\u201d class due to the possible cross-hypersensitivity reactions with thiomersal.
\nConclusion: The frequency of positive thiomersal reactions in our patients (10%) would justify its introduction into the Additional Baseline Series. Additional studies including a higher number of patients are needed to clarify the importance of genetic polymorphisms in thiomersal contact allergy. <\/p>\n<\/span><\/p>\n
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